Now showing items 1-9 of 9

    • A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction 

      González Viacava, Myriam Belén; Leiva, Carlos Leónidas; Tavarone, Maria Eugenia; Dokmetjian, José Christian; Cascone, Osvaldo; Chacana, Pablo; Fingermann, Matías (Elsevier, 2022-11)
      Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal ...
    • Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies 

      Bellido, Demian; Chacana, Pablo; Mozgovoj, Marina Valeria; Gonzalez, Diego; Goldbaum, Fernando Alberto; Wigdorovitz, Andres; Dus Santos, Maria Jose (Scientific Research, 2012-02)
      Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but ...
    • Chicken egg yolk antibodies against bovine respiratory syncytial virus neutralize the virus in vitro 

      Ferella, Alejandra; Bellido, Demian; Chacana, Pablo; Wigdorovitz, Andres; Dus Santos, Maria Jose; Mozgovoj, Marina Valeria (Elsevier, 2012)
      Bovine respiratory syncytial virus (BRSV) and its counterpart in humans (HRSV) are two closely related virus, which are the leading cause of severe respiratory syndrome in calves and young children, respectively. Passive ...
    • IgY technology: producing antibodies from a 3Rs perspective 

      Leiva, Carlos Leónidas; Casanova, Natalia Andrea; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo (2018-07)
    • IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy 

      Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia; Farace, Mariano; Geoghegan, Patricia; Brero, Luisa; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo Anibal (Elsevier, 2019-05)
      Antivenom for the treatment of bothropic snakebite is a priority for public health institutions from Latin America. An alternative to the conventional antivenom production is based on the use of egg yolk antibodies – ...
    • IgY-technology (egg yolk antibodies) in human medicine: a review of patents and clinical trials 

      Leiva, Carlos; Gallardo, Mauro Julián; Casanova, Natalia Andrea; Terzolo, Horacio; Chacana, Pablo (Elsevier, 2020-04)
      IgY-technology (the production and extraction of specific IgY antibodies from egg yolk) is an innovative method to produce antibodies for therapy and prophylaxis. Advantages of IgY over other antibodies comprise its ...
    • In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses 

      Bustos, Carla Paola; Leiva, Carlos Leónidas; Gambarotta, Mariana; Guida, Nora; Chacana, Pablo (Elsevier, 2021-08)
      Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant ...
    • In vivo neutralization of bee venom lethality by IgY antibodies 

      Leiva, Carlos Leónidas; Geoghegan, Patricia; Lammer, Mónica; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Brero, Luisa; Chacana, Pablo (Elsevier, 2021-07)
      Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings ...
    • Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom 

      Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia; Celi Castillo, Ana Beatriz; Joaquim, Patricia Estefania; Geoghegan, Patricia; Fernandez Miyakawa, Mariano Enrique; Chacana, Pablo (Springer, 2023-02)
      The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants ...