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Mostrando ítems 1-20 de 114
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A beginner’s guide for FMDV quasispecies analysis: sub-consensus variant detection and haplotype reconstruction using next-generation sequencing
(Oxford Academic Press, 2020-09)Deep sequencing of viral genomes is a powerful tool to study RNA virus complexity. However, the analysis of next-generation sequencing data might be challenging for researchers who have never approached the study of viral ... -
AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
(Plos One, 2012-09)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ... -
Aislamiento y caracterización de regiones promotoras de girasol para dirigir la expresión tejido específica de genes foráneos a semilla
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2014-04)Desde el inicio de la utilización de variedades transgénicas, la superficie agrobiotecnológica ha aumentado exponencialmente, con un incremento del orden de 80 veces en solo 14 años, y su uso se ha expandido a más de veinte ... -
Análisis funcional del factor de transcripción ASR1 desde una perspectiva metabólica, fisiológica y molecular en plantas de tabaco y tomate
(Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 2014-11)La familia de genes Asr (ABA, stress, ripening) está implicada en las vías de respuestas generales a estrés y en el control del transporte de hexosas. Para profundizar en el estudio funcional del parálogo ASR1 en Solanáceas, ... -
Análisis genómico de girasol: desarrollo de colecciones de ESTs y de una plataforma bioinformática para estudios de expresión de genes candidato en respuestas a estreses abióticos
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2006)El girasol es uno de los principales cultivos oleaginosos del mundo por su volumen de producción y composición en ácidos grasos insaturados. En promedio, durante los últimos cinco años, la producción mundial se ha mantenido ... -
Aplicaciones y perspectivas de la edición génica de plantas mediante CRISPR-Cas9 = Applications and perspectives of plant gene editing using CRISPR-Cas9
(Universidad de Morón, 2022)Los avances en materia de genética y biotecnología, introducen una dimensión novedosa en la utilización de herramientas de edición génica en plantas. En el presente trabajo se relevaron 164 publicaciones científicas que ... -
Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
(2014-04)RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors ... -
Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads
(2015-08)Tumor necrosis factor alpha (TNF-α) is a pleiotropic cytokine involved in the immune response against viral and other infections. Its expression levels are affected by a polymorphism in the promoter region of the gene. ... -
BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
(2017-05)Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of ... -
Candidate Gene Approach for Parasite Resistance in Sheep – Variation in Immune Pathway Genes and Association with Fecal Egg Count
(Plos One, 2014-02-12)Sheep chromosome 3 (Oar3) has the largest number of QTLs reported to be significantly associated with resistance to gastro-intestinal nematodes. This study aimed to identify single nucleotide polymorphisms (SNPs) within ... -
Caracterización molecular de mutantes derivadas del genotipo mutador de cloroplastos de cebada
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2008)La coordinación de la expresión génica entre los genomas nuclear, plastídico y mitocondrial es de importancia crucial para las células vegetales. Un caso particular de la interacción núcleo-plástido está dado por los genes ... -
Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment
(2012-08)Phenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In ... -
Científicos secuencian el genoma del tomate
(Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2011-04)Un consorcio internacional, en el que participa el INTA y otras instituciones nacionales, logró identificar el código genético de este cultivo. El hallazgo permitirá implementar estrategias de mejoramiento para optimizar ... -
Construction and manipulation of a full-length infectious bacterial artificial chromosome clone of equine herpesvirus type 3 (EHV-3)
(2017-01)Equine herpesvirus type 3 (EHV-3) is the causal agent of equine coital exanthema, a disease characterizedby pox-like lesions on the penis of stallions and the vulva of mares. Although the complete genomicsequence of EHV-3 ... -
CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
(Springer, 2023-02)Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ... -
Cytogenetic analysis of the South American fruit fly Anastrepha fraterculus (diptera:tephritidae) species complex : construction of detailed photographic polytene chromosome maps of the Argentinian af. sp.1 member
(2016)Genetic and cytogenetic studies constitute a significant basis for understanding the biology of insect pests and the design and the construction of genetic tools for biological control strategies. Anastrepha fraterculus ... -
Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages
(Elsevier, 2023-05)Brucella parasitize the macrophage where is able to replicate and modulate the immune response in order to establish a chronic infection. The most adequate response to control and eliminate Brucella infection is a type 1 ... -
Data on polyphenols and biological activity analyses of an Andean tomato collection and their relationships with tomato traits and geographical origin
(2016)Data provide information about a tomato collection composed of accessions from the Andean Valley, commercial accessions and wild species. Antioxidant metabolites were measured inmature fruits of this collection, and their ... -
Dataset on absorption spectra and bulb concentration of phenolic compounds that may interfere with onion pyruvate determinations
(2017)We present data on absorption spectra (400–540 nm) and concentration of phenolic compounds quercetin, myricetin, kaempferol, rutin, catechin, epicatechin gallate(ECG) and epigallocatechin gallate (EGCG), inyellow, redand ... -
Deep cytogenetics analysis reveals meiotic recombination depletion in species of Senecio (Asteraceae)
(Springer Open, 2013-08)Background: Senecio is the largest genus in the Asteraceae family growing in all environments around the world. It displays taxonomic and systematical difficulties. Cytogenetic knowledge of this genus is ancient, scarce ...