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Mostrando ítems 310-329 de 1219
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Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
(Elsevier, 2020-02)Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving ... -
Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
(Springer, 2023-04)Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of ... -
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19
(Elsevier, 2022-01)Serology tests for SARS-CoV-2 have proven to be important tools to fight against the COVID-19 pandemic. These serological tests can be used in low-income and remote areas for patient contact tracing, epidemiologic studies ... -
Development and evaluation of a new lateral flow assay for simultaneous detection of antibodies against African horse sickness and equine infectious anemia viruses
(2016)African horse sickness (AHS) and equine infectious anemia (EIA) are both notifiable equid specific diseases that may present similar clinical signs. Considering the increased global movement of horses and equine products ... -
Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples
(BMC, 2019-04)Background: Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and has a negative impact on equine breeding enterprises worldwide. Among ERVA strains infecting foals, the genotypes G3P[12] and ... -
Development and predation of Tupiocoris cucurbitaceus feeding on Tetranychus urticae
(Taylor & Francis, 2021-03-16)We evaluated the predatory bug, Tupiocoris cucurbitaceus as biocontrol agent of the two spotted spider mite, Tetranychus urticae, by analysing its predatory capacity, development to adult stage when fed on this pest, and ... -
Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
(Annex Publishers, 2019-08-26)Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several ... -
Development of a construct-based risk assessment framework for genetic engineered crops
(2016)Experience gained in the risk assessment (RA) of genetically engineered (GE) crops since their first experimental introductions in the early nineties, has increased the level of familiarity with these breeding methodologies ... -
Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
(Elsevier, 2020-01)Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is ... -
Development of a droplet digital PCR assay for quantification of the proviral load of bovine leukemia virus
(Sage Publications, 2022-05)Droplet digital PCR (ddPCR) is a highly sensitive tool developed for the detection and quantification of short-sequence variants—a tool that offers unparalleled precision enabling measurement of smaller-fold changes. We ... -
Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
(Elsevier, 2021-04)Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection ... -
Development of a loop-mediated isothermal amplification (LAMP) and a direct LAMP for the specific detection of Nosema ceranae, a parasite of honey bees
(Springer, 2020-10)Nosema ceranae is a ubiquitous microsporidian pathogen infecting the midgut of honey bees. The infection causes bee nosemosis, a disease associated with malnutrition, dysentery, and lethargic behavior, and results in ... -
Development of a Multilocus Sequence Typing scheme for the study of Anaplasma marginale population structure over space and time
(Elsevier, 2015-03)Bovine Anaplasmosis caused by Anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where Rhipicephalus microplus is considered the most significant biological vector. Molecular markers ... -
Development of a novel set of Gateway-compatible vectors for live imaging in insect cells
(The Royal Entomological Society, 2011-10)Insect genomics is a growing area of research. To exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. ... -
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
(2017-12)Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have ... -
Development of a platform process for the production and purification of single‐domain antibodies
(Wiley, 2021-09)Single-domain antibodies (sdAbs) offer the affinity and therapeutic value of conventional antibodies, with increased stability and solubility. Unlike conventional antibodies, however, sdAbs do not benefit from a platform ... -
Development of a Recombination System for the Generation of Occlusion Positive Genetically Modified Anticarsia Gemmatalis Multiple Nucleopolyhedrovirus
(2015)Anticarsia gemmatalis is an important pest in legume crops in South America and it has been successfully controlled using Anticarsia gemmatalis Multiple Nucleopolyhedrovirus(AgMNPV) in subtropical climate zones. Nevertheless, ... -
Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2
(Elsevier, 2015-09)The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting ... -
Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves
(MDPI, 2023-03)Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific ... -
Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
(2018)The current method for Babesia spp. serodiagnosis based on a crude merozoite antigen is a complex and time-consuming procedure. An indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant multi-antigen ...