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Abstract
Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be [ver mas...]
dc.contributor.authorGodoy, Fabiana de
dc.contributor.authorBermúdez, Luisa
dc.contributor.authorLira, Bruno Silvestre
dc.contributor.authorSouza, Amanda Pereira de
dc.contributor.authorElbl, Paula
dc.contributor.authorDemarco, Diego
dc.contributor.authorAlseekh, Saleh
dc.contributor.authorInsani, Ester Marina
dc.contributor.authorBuckeridge, Marcos
dc.contributor.authorAlmeida, Juliana
dc.contributor.authorGrigioni, Gabriela Maria
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorCarrari, Fernando
dc.contributor.authorRossi, Magdalena
dc.date.accessioned2017-11-02T13:04:18Z
dc.date.available2017-11-02T13:04:18Z
dc.date.issued2013-04
dc.identifier.issn0022-0957 (Print)
dc.identifier.issn1460-2431 (Online)
dc.identifier.otherhttps://doi.org/10.1093/jxb/ert106
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1655
dc.identifier.urihttps://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert106
dc.description.abstractPectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomatoeng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceJournal of Experimental Botany 64 (8) : 2449–2466 (May 2013)
dc.subjectTomatees_AR
dc.subjectTomatoeseng
dc.subjectPectinases_AR
dc.subjectPectinseng
dc.subjectCarbono
dc.subjectCarboneng
dc.subject.otherGalacturonosyltransferase
dc.titleGalacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomatoes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.filFil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Bermúdez, Luisa. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Souza, Amanda Pereira de. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Elbl, Paula. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Alseekh, Saleh. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Buckeridge, Marcos. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.description.filFil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina
dc.description.filFil: Fernie, Alisdair Robert. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica; Brasil
dc.subtypecientifico


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