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Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed [ver mas...]
dc.contributor.authorDistefano, Ana Julia
dc.contributor.authorMaldonado, Sara Beatriz
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorDel Vas, Mariana
dc.date.accessioned2023-09-28T15:19:34Z
dc.date.available2023-09-28T15:19:34Z
dc.date.issued2009-06
dc.identifier.issn1572-994X
dc.identifier.otherhttps://doi.org/10.1007/s11262-009-0345-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/15366
dc.identifier.urihttps://link.springer.com/article/10.1007/s11262-009-0345-2
dc.description.abstractMal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/es_AR
dc.sourceVirus Genes 38 (3) : 455-460 (Junio 2009)es_AR
dc.subjectFijiviruses_AR
dc.subjectMaizeeng
dc.subjectMaízes_AR
dc.subjectStructural Proteinseng
dc.subjectProteínas Estructuraleses_AR
dc.subject.otherMal de Río Cuarto Viruseng
dc.subject.otherVirus del Mal de Río Cuartoes_AR
dc.subject.otherCapsideng
dc.subject.otherCápsidees_AR
dc.titleMal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid proteines_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)es_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Distefano, Ana Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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