Temas
Mostrando ítems 41-57 de 57
-
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
(Elsevier, 2018-07)Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are ... -
Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters
(Springer, 2024-03)Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated ... -
Pandemic (H1N1) 2009 outbreak on pig farm, Argentina
(Centers for Disease Control and Prevention, 2010-02)In June–July 2009, an outbreak of pandemic (H1N1) 2009 infection occurred on a pig farm in Argentina. Molecular analysis indicated that the virus was genetically related to the pandemic (H1N1) 2009 influenza virus strain. ... -
Preparation of a working seed lot of BCG and quality control by PCR genotyping
(Asociación Argentina de Microbiología, 2010-04)The bacillus Calmette-Guérin (BCG) was obtained in 1920 after successive passages leading to the attenuation of a Mycobacterium bovis strain. For the following 40 years, BCG had been replicated, resulting in substrains ... -
Primer reporte de Sweet potato leaf curl virus en las provincias de Córdoba y Santiago del Estero de Argentina
(Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, 2016-03)La superficie con batata en Argentina experimentó notable reducción, causada por virosis que ocasionan daños en todas las regiones productoras, debido al intercambio indiscriminado de material de propagación y consiguiente ... -
Puesta a punto de una PCR en tiempo real para la detección de Senecavirus A
(Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)Senecavirus A (SVA). Familia : Picornaviridae Única especie del género Senecavirus. No envuelto ARN + monocatenario. La Organización Mundial de Sanidad Animal ( lo considera de gran importancia debido a que sus signos ... -
Quadruplex real-time TaqMan® RT-qPCR assay for differentiation of equine group A and B rotaviruses and Identification of group A G3 and G14 genotypes
(MDPI, 2023-07)Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a ... -
Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
(Elsevier, 2013-10)In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing ... -
Rapid detection of equine infectious anaemia virus nucleic acid by insulated isothermal RT-PCR assay to aid diagnosis under field conditions
(Wiley, 2018)Background: Control of equine infectious anaemia (EIA) currently depends on serological diagnosis of infected equids. However, recently infected equids may not produce detectable anti-EIAV antibodies up to 157 days post ... -
Tuberculosis bovina : transmisibilidad de cepas de Mycobacterium bovis y detección de micobacterias en menudencias comercializadas en bocas de expendio de carne
(Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2019)La tuberculosis bovina, causada por Mycobacterium bovis (M. bovis), es endémica en la Argentina. Su presencia en el ganado bovino representa un riesgo para la salud humana debido a su carácter zoonótico, pudiendo además ... -
Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina
(Cambridge University Press, 2015-04)In Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics of Mycobacterium avium complex (MAC) infection in a swine population of Argentina ... -
Use of 454-Pyrosequencing for the characterization of Sweet potato virus C and Sweet Potato Feathery Mottle virus Isolates fron Argentina and Development of a Multiplex one - step RT-PCR for Their Simultaneous Detection
(2016)454-pyrosequencing was applied as a tool to identify etiological agents involved in the symptoms observed in a sweet potato sample from Argentina. RNA was purified from symptomatic material and sequenced using a Roche 454 ... -
Use of Chelex-100 for the molecular diagnosisof five animal pathogens
(Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-06)Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) ... -
Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
(Elsevier, 2021-10)Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we ... -
Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
(Sage Publications, 2005-05)The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and ... -
Validation of reference genes for quantitative PCR in Johnsongrass (Sorghum halepense L.) under glyphosate stress
(MDPI, 2021-08)Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. ... -
Variabilidad en cepas de Xylella fastidiosa procedentes de olivo (Olea europaea L.) de Argentina [resumen]
(Sociedad Argentina de Biología, 2020-09-09)Xylella fastidiosa, es considerada una bacteria polífaga, capaz de vivir y multiplicarse en un amplio rango de hospedantes, más de 500 especies de plantas, abarcando tanto cultivos de interés agrícola como especies ...