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Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia [ver mas...]
dc.contributor.authorPérez, Matías
dc.contributor.authorMacchiaroli, Natalia
dc.contributor.authorLichtenstein, Gabriel
dc.contributor.authorConti, Gabriela
dc.contributor.authorAsurmendi, Sebastian
dc.contributor.authorMilone, Diego Humberto
dc.contributor.authorStegmayer, Georgina
dc.contributor.authorKamenetzky, Laura
dc.contributor.authorCucher, Marcela
dc.contributor.authorRosenzvit, Mara Cecilia
dc.date.accessioned2017-10-11T13:35:35Z
dc.date.available2017-10-11T13:35:35Z
dc.date.issued2017-09
dc.identifier.issn0020-7519 (Print)
dc.identifier.issn1879-0135 (Online)
dc.identifier.otherhttps://doi.org/10.1016/j.ijpara.2017.04.002
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1458
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0020751917301315?via%3Dihub
dc.description.abstractMicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T. solium genome. Moreover, we analysed the effect of praziquantel, one of the two main drugs used for cysticercosis treatment, on the miRNA expression profile of T. crassiceps cysticerci. Using small RNA-seq and two independent algorithms for miRNA prediction, as well as northern blot validation, we found transcriptional evidence of 39 miRNA loci in T. crassiceps. Since miRNAs were mapped to the T. solium genome, these miRNAs are considered common to both parasites. The miRNA expression profile of T. crassiceps was biased to the same set of highly expressed miRNAs reported in other cestodes. We found a significant altered expression of miR-7b under praziquantel treatment. In addition, we searched for miRNAs predicted to target genes related to drug response. We performed a detailed target prediction for miR-7b and found genes related to drug action. We report an initial approach to study the effect of sub-lethal drug treatment on miRNA expression in a cestode parasite, which provides a platform for further studies of miRNA involvement in drug effects. The results of our work could be applied to drug development and provide basic knowledge of cysticercosis and other neglected helminth infections.eng
dc.formatapplication/pdf
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceInternational journal for parasitology 47 (10–11) : 643-653 (September 2017)
dc.subjectGenética
dc.subjectGeneticseng
dc.subjectARN
dc.subjectRNAeng
dc.subjectTaenia Crassiceps
dc.subjectTaenia Solium
dc.subjectIdentificación
dc.subjectIdentificationeng
dc.titleMicroRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/acceptedVersion
dc.description.origenInst. de Biotecnología
dc.gic154987
dc.description.filFil: Pérez, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina
dc.description.filFil: Macchiaroli, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina
dc.description.filFil: Lichtenstein, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina
dc.description.filFil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Milone, Diego Humberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Sistemas e Inteligencia Computacion. Instituto de Investigación en Señales; Argentina
dc.description.filFil: Stegmayer, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Sistemas e Inteligencia Computacion. Instituto de Investigación en Señales; Argentina
dc.description.filFil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.filFil: Cucher, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina;Argentina
dc.description.filRosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.subtypecientifico


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