Analysis of DNA Extraction Protocols for Transgenic Events Determination in Alfalfa

Abstract

Alfalfa (Medicago sativa) is one of the main forage crops in Argentina. The unclear introduction of GE glyphosate-resistant alfalfa seeds, caused contamination in different production areas, affecting the purity of the local non-GE alfalfa seeds. Also, that contamination could affect the international trade of forage to countries where such transgenic events are prohibited. The evaluation of these events could be performed by DNA evaluation. In this sense, it is essential to have methodologies that allow us to obtain a good DNA concentration and quality.