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Resumen
The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and
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dc.contributor.author | Arizio, Carla Marcela | |
dc.contributor.author | Costa Tártara, Sabrina María | |
dc.contributor.author | Zunino, Ignacio | |
dc.contributor.author | Manifesto, Maria Marcela | |
dc.date.accessioned | 2022-12-22T10:22:13Z | |
dc.date.available | 2022-12-22T10:22:13Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0001-3765 | |
dc.identifier.issn | 1678-2690 | |
dc.identifier.other | https://doi.org/10.1590/0001-3765202220210672 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/13679 | |
dc.identifier.uri | https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en | |
dc.description.abstract | The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Academia Brasileira de Ciencias | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022) | es_AR |
dc.subject | Anthocyanins | eng |
dc.subject | Antocianinas | es_AR |
dc.subject | Candidate Genes | eng |
dc.subject | Genes Candidatos | es_AR |
dc.subject | Genetic Diversity (as resource) | eng |
dc.subject | Diversidad Genética (como recurso) | es_AR |
dc.subject | Ipomoea | |
dc.subject | Marcadores Genéticos | |
dc.subject | Genetic Markers | eng |
dc.subject.other | ILP-CG Markers | eng |
dc.subject.other | Marcadores ILP-CG | es_AR |
dc.subject.other | Wild Ipomoeas | eng |
dc.subject.other | Ipomoeas Salvajes | es_AR |
dc.title | Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.fil | Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina | es_AR |
dc.description.fil | Fil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; Argentina | es_AR |
dc.description.fil | Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina | es_AR |
dc.subtype | cientifico |
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