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Abstract
Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 [ver mas...]
dc.contributor.authorSouza, Mariana Assunção de
dc.contributor.authorLopes, Thaís Aline Carolino
dc.contributor.authorSilva, Brendhal Almeida
dc.contributor.authorBombonato, Nadia Grandi
dc.contributor.authorDib, Cristina Corsi
dc.contributor.authorMarfil, Maria Jimena
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorLima, Anna Monteiro Correia
dc.date.accessioned2022-08-04T11:29:23Z
dc.date.available2022-08-04T11:29:23Z
dc.date.issued2022-07
dc.identifier.issn1573-7438
dc.identifier.otherhttps://doi.org/10.1007/s11250-022-03243-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12493
dc.identifier.urihttps://link.springer.com/article/10.1007/s11250-022-03243-2
dc.description.abstractGenotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicaes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceTropical Animal Health and Production 54 (4) : 238 (Julio 2022)es_AR
dc.subjectEpidemiologyeng
dc.subjectEpidemiologíaes_AR
dc.subjectBovinaees_AR
dc.subjectTuberculosiseng
dc.subjectMycobacterium bovises_AR
dc.subjectCattleeng
dc.subjectGanado Bovinoes_AR
dc.subjectGenotypingeng
dc.subjectGenotipadoes_AR
dc.subjectBrazileng
dc.subjectBrasiles_AR
dc.subject.otherSpoligotypeseng
dc.subject.otherEspoligotiposes_AR
dc.titleSpatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Braziles_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: de Souza, Mariana Assunção. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasiles_AR
dc.description.filFil: Lopes, Thaís Aline Carolino. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasiles_AR
dc.description.filFil: Silva, Brendhal Almeida. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasiles_AR
dc.description.filFil: Bombonato, Nadia Grandi. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasiles_AR
dc.description.filFil: Dib, Cristina Corsi. Instituto Biológico de São Paulo. Laboratório de Tuberculose; Brasiles_AR
dc.description.filFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Lima, Anna Monteiro Correia. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasiles_AR
dc.subtypecientifico


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