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Abstract
Valorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other polysaccharides by secreting a complex array of enzymes. In this work, we studied the specificity and synergy of two enzymes, CsXyn10A and CsAbf62A, which were identified as highly abundant in the extracellular proteome of Cellulomonas [ver mas...]
dc.contributor.authorGarrido, Mercedes Maria
dc.contributor.authorPiccinni, Florencia Elizabeth
dc.contributor.authorLandoni, Malena
dc.contributor.authorPeña, María Jesús
dc.contributor.authorTopalian, Juliana
dc.contributor.authorCouto, Alicia
dc.contributor.authorWirth, Sonia Alejandra
dc.contributor.authorUrbanowicz, Breeanna Rae
dc.contributor.authorCampos, Eleonora
dc.date.accessioned2022-07-27T11:10:21Z
dc.date.available2022-07-27T11:10:21Z
dc.date.issued2022
dc.identifier.issn1432-0614
dc.identifier.otherhttps://doi.org/10.1007/s00253-022-12061-3
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12415
dc.identifier.urihttps://link.springer.com/article/10.1007/s00253-022-12061-3
dc.description.abstractValorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other polysaccharides by secreting a complex array of enzymes. In this work, we studied the specificity and synergy of two enzymes, CsXyn10A and CsAbf62A, which were identified as highly abundant in the extracellular proteome of Cellulomonas sp. B6 when grown on wheat bran. To explore their potential for bioprocessing, the recombinant enzymes were expressed and their activities were thoroughly characterized. rCsXyn10A is a GH10 endo-xylanase (EC 3.2.1.8), active across a broad pH range (5 to 9), at temperatures up to 55 °C. rCsAbf62A is an α-L-arabinofuranosidase (ABF) (EC 3.2.1.55) that specifically removes α-1,2 and α-1,3-L-arabinosyl substituents from arabino-xylo-oligosaccharides (AXOS), xylan, and arabinan backbones, but it cannot act on double-substituted residues. It also has activity on pNPA. No differences were observed regarding activity when CsAbf62A was expressed with its appended CBM13 module or only the catalytic domain. The amount of xylobiose released from either wheat arabinoxylan or arabino-xylo-oligosaccharides increased significantly when rCsXyn10A was supplemented with rCsAbf62A, indicating that the removal of arabinosyl residues by rCsAbf62A improved rCsXyn10A accessibility to β-1,4-xylose linkages, but no synergism was observed in the deconstruction of wheat bran. These results contribute to designing tailor-made, substrate-specific, enzymatic cocktails for xylan valorization.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E7-I152-001/2019-PD-E7-I152-001/AR./Alimentos nutracéuticos, funcionales o para regímenes especiales.es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PE-E7-I149-001/2019-PE-E7-I149-001/AR./Bioenergía generada en origen como aporte al desarrollo territoriales_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceApplied Microbiology and Biotechnology (Published: 08 July 2022)es_AR
dc.subjectHemicelluloseeng
dc.subjectHemicelulosaes_AR
dc.subjectCellulomonases_AR
dc.subjectXylanseng
dc.subjectXilanoses_AR
dc.titleInsights into the xylan degradation system of Cellulomonas sp. B6 : biochemical characterization of rCsXyn10A and rCsAbf62Aes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Garrido, Mercedes Maria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Piccinni, Florencia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Piccinni, Florencia Elizabeth. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Landoni, Malena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono; Argentinaes_AR
dc.description.filFil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Peña, María Jesús. University of Georgia. Complex Carbohydrate Research Center; Estados Unidoses_AR
dc.description.filFil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Couto, Alicia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Hidratos de Carbono; Argentinaes_AR
dc.description.filFil: Wirth, Sonia Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.description.filFil: Wirth, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Urbanowicz, Breeanna Rae. University of Georgia. Department of Biochemistry and Molecular Biology; Estados Unidoses_AR
dc.description.filFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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