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Abstract
Cultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques [ver mas...]
dc.contributor.authorSalvador, Ricardo
dc.contributor.authorMaskin, Laura
dc.contributor.authorNiz, José María
dc.contributor.authorTurica, Mariana Daniela
dc.contributor.authorPedarros, Analía Soledad
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorLewi, Dalia Marcela
dc.date.accessioned2022-07-26T22:43:52Z
dc.date.available2022-07-26T22:43:52Z
dc.date.issued2022
dc.identifier.citationSalvador, R. et al. (2022). RNAi Expression in Cotton for Control of Herbivorous Insects. pp 217-233. In: Vaschetto, L.M. (eds) RNAi Strategies for Pest Management. Methods in Molecular Biology, vol 2360. Humana, New York, NYes_AR
dc.identifier.isbn978-1-0716-1633-8
dc.identifier.otherhttps://doi.org/10.1007/978-1-0716-1633-8_17
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12411
dc.identifier.urihttps://link.springer.com/protocol/10.1007/978-1-0716-1633-8_17
dc.description.abstractCultivated cotton (Gossypium hirsutum) is heavily attacked by various species of insects worldwide and breeding of new varieties resistant to pests is still a hard battle to win. RNAi technology is an important reverse genetics tool to induce gene silencing in eukaryotic organisms and produce phenotypic modifications. In cotton, RNAi was applied to investigate gene function and enhance resistance to insects and pathogens. Different methods and techniques can be used to synthetize double stranded RNA (dsRNA) into plant cells. The Agrobacterium-mediated transformation is a common method to introduce RNAi binary plasmids into cotton genome and obtain stable transgenics plants. This methodology includes the coculture of cotton tissues with Agrobacterium cultures, selection of transgenic cells and induction of somatic embryogenesis to finally obtain transgenic plants after a relatively long period of time. The transient synthesis of dsRNA mediated by virus-induced gene silencing (VIGS) in cotton is an alternative to anticipate the silencing effect of a specific RNA sequence, prior to the development of a stable transgenic plant. VIGS vectors are incorporated into the plant by agroinfiltration technique. During VIGS replication inside plant cells, synthetized dsRNA allows the study on specific heterologous gene expression including the phenotypic effect on herbivorous target pests, thus facilitating a rapid evaluation of dsRNA expressed in cotton plants against individual insect target genes. Here we describe the complementation of these two techniques to evaluate RNAi-based cotton plant protection against insect pests.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.relation.ispartofseriesMethods in Molecular Biology (Springer)es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceRNAi Strategies for Pest Management : methods and protocols / editors: Luis María Vaschetto. New York: Humana, 2021. p. 217–233es_AR
dc.subjectGossypium hirsutumes_AR
dc.subjectAlgodónes_AR
dc.subjectCottoneng
dc.subjectControl Biológico
dc.subjectBiological Controleng
dc.subjectControl de Plagas
dc.subjectPest Controleng
dc.subject.otherTransgenic Cotton Plantseng
dc.subject.otherVirus Induced Gene Silencingeng
dc.subject.otherRNA Interferenceeng
dc.titleRNAi Expression in Cotton for Control of Herbivorous Insectses_AR
dc.typeinfo:ar-repo/semantics/parte de libroes_AR
dc.typeinfo:eu-repo/semantics/bookPartes_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Microbiología y Zoología Agrícola (IMYZA)es_AR
dc.description.filFil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Maskin, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Niz, José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Turica, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Pedarros, Analía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentinaes_AR
dc.description.filFil: Lewi, Dalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.subtypelibro


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