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Background and Aims: Plants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular patterns (MAMPs or PAMPs, respectively) by membrane-bound receptors. Indeed, PTI restricts virus infection in plants and, in addition, BRI1-associated kinase 1 (BAK1), a central regulator of PTI, plays a role in antiviral resistance. [ver mas...]
dc.contributor.authorCaro, Maria Del Pilar
dc.contributor.authorVenturuzzi, Andrea Laura
dc.contributor.authorMoschen, Sebastian Nicolas
dc.contributor.authorSalazar, Sergio Miguel
dc.contributor.authorDíaz Ricci, Juan Carlos
dc.contributor.authorAsurmendi, Sebastian
dc.date.accessioned2022-07-07T10:48:16Z
dc.date.available2022-07-07T10:48:16Z
dc.date.issued2022-04
dc.identifier.issn0305-7364
dc.identifier.otherhttps://doi.org/10.1093/aob/mcac013
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12262
dc.identifier.urihttps://academic.oup.com/aob/article-abstract/129/5/593/6522799
dc.description.abstractBackground and Aims: Plants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular patterns (MAMPs or PAMPs, respectively) by membrane-bound receptors. Indeed, PTI restricts virus infection in plants and, in addition, BRI1-associated kinase 1 (BAK1), a central regulator of PTI, plays a role in antiviral resistance. However, the compounds that trigger antiviral defences, along with their molecular mechanisms of action, remain mostly elusive. Herein, we explore the role of a fungal extracellular subtilase named AsES in its capacity to trigger antiviral responses. Methods: In this study, we obtained AsES by recombinant expression, and evaluated and characterized its capacity to trigger antiviral responses against Tobacco mosaic virus (TMV) by performing time course experiments, analysing gene expression, virus movement and callose deposition. Key Results: The results of this study provide direct evidence that exogenous treatment with recombinant AsES increases a state of resistance against TMV infection, in both arabidopsis and Nicotiana benthamiana plants. Also, the antiviral PTI response exhibited by AsES in arabidopsis is mediated by the BAK1/SERK3 and BKK1/SERK4 co-receptors. Moreover, AsES requires a fully active salicylic acid (SA) signalling pathway to restrict the TMV movement by inducing callose deposition. Additionally, treatment with PSP1, a biostimulant based on AsES as the active compound, showed an increased resistance against TMV in N. benthamiana and tobacco plants. Conclusions: AsES is a fungal serine protease which triggers antiviral responses relying on a conserved mechanism by means of the SA signalling pathway and could be exploited as an effective and sustainable biotechnology strategy for viral disease management in plants.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherOxford University Presses_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.es_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceAnnals of Botany 129 (5) : 593-606 (Abril 2022)es_AR
dc.subjectProteaseseng
dc.subjectProteasases_AR
dc.subjectTobacco Mosaic Tobamoviruseng
dc.subjectTobamovirus del Mosaico del Tabacoes_AR
dc.subjectImmunityeng
dc.subjectInmunidades_AR
dc.subjectSalicylic Acidseng
dc.subjectÁcido Salicílicoes_AR
dc.subjectImmune Responseeng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectArabidopsiseng
dc.subjectNicotianaeng
dc.subject.otherNicotiana benthamianaes_AR
dc.subject.otherPlant Immunityeng
dc.subject.otherInmunidad Vegetales_AR
dc.titleA fungal protease named AsES triggers antiviral immune responses and effectively restricts virus infection in arabidopsis and Nicotiana benthamiana plantses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Caro, María del Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Caro, María del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Caro, María del Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentinaes_AR
dc.description.filFil: Venturuzzi, Andrea Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Venturuzzi, Andrea Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentinaes_AR
dc.description.filFil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentinaes_AR
dc.description.filFil: Diaz Ricci, Juan Carlos. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentinaes_AR
dc.description.filFil: Diaz Ricci, Juan Carlos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica Dr. Bernabé Bloj; Argentinaes_AR
dc.description.filFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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