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Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority.Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we [ver mas...]
dc.contributor.authorVarni, Vanina Delia
dc.contributor.authorCaimi, Karina Cynthia
dc.contributor.authorJacob, Paulina
dc.contributor.authorChiani, Yosena
dc.contributor.authorLandolt, Noelia
dc.contributor.authorSchmeling, María Fernanda
dc.contributor.authorPujato, Nazarena
dc.contributor.authorVanasco, Bibiana
dc.coverage.spatialArgentina (nation)
dc.date.accessioned2017-09-11T14:46:23Z
dc.date.available2017-09-11T14:46:23Z
dc.date.issued2016
dc.identifier.issn1567-1348 (Print)
dc.identifier.issn1567-7257 (Online)
dc.identifier.otherhttps://doi.org/10.1016/j.meegid.2015.11.033
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1192
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S1567134815300630
dc.description.abstractLeptospira typing is carried out using isolated strains. Because of difficulties in obtaining them, direct identification of infective Leptospira in clinical samples is a high priority.Multilocus sequence typing (MLST) proved highly discriminatory for seven pathogenic species of Leptospira, allowing isolate characterization and robust assignment to species, in addition to phylogenetic evidence for the relatedness between species. In this study we characterized Leptospira strains circulating in Argentina, using typing methods applied to human clinical samples and isolates. Phylogenetic studies based on 16S ribosomal RNA gene sequences enabled typing of 8 isolates (6 Leptospira interrogans, one Leptospirawolffii and one Leptospira broomii) and 58 out of 85 (68.2%) clinical samples (55 L. interrogans, 2 Leptospira meyeri, and one Leptospira kirschneri). MLST results for the L. interrogans isolates indicated that five were probably Canicola serogroup (ST37) and one was probably Icterohaemorrhagiae serogroup (ST17). Eleven clinical samples (21.6%), provided MLST interpretable data: five were probably Pyrogenes serogroup (ST13), four Sejroe (ST20), one Autumnalis (ST22) and one Canicola (ST37). To the best of our knowledge this study is the first report of the use of an MLST typing scheme with seven loci to identify Leptospira directly from clinical samples in Argentina. The use of clinical samples presents the advantage of the possibility of knowing the infecting strain without resorting to isolates. This study also allowed, for the first time, the characterization of isolates of intermediate pathogenicity species (L. wolffii and L. broomii) from symptomatic patients.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceInfection, genetics and evolution 37 : 245-251. (January 2016)eng
dc.subjectLeptospirosis
dc.subjectEnfermedades Humanas
dc.subjectHuman Diseaseseng
dc.subjectEnsayos Clínicos
dc.subjectClinical Trialseng
dc.subjectTécnicas de Aislamiento
dc.subjectIsolation Techniqueseng
dc.titleIsolation and clinical sample typing of human leptospirosis cases in Argentinaeng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.origenInst. de Biotecnología
dc.gic368
dc.description.filFil: Varni, Vanina Delia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Jacob, Paulina. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
dc.description.filFil: Landolt, Noelia. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
dc.description.filFil: Schmeling, María Fernanda. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
dc.description.filFil: Pujato, Nazarena. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
dc.description.filFil: Vanasco, Bibiana. Instituto Nacional de Enfermedades Respiratorias “Dr. E. Coni”; Argentina. Administración Nacional de Laboratorios e Institutos de Salud; Argentina
dc.subtypecientifico


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