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Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde [ver mas...]
dc.contributor.authorColuccio Leskow, Carla
dc.contributor.authorDominguez, Pia Guadalupe
dc.contributor.authorDíaz Zirpolo, José Antonio
dc.contributor.authorObata, Toshihiro
dc.contributor.authorKamenetzky, Laura
dc.contributor.authorCosta, Hernán
dc.contributor.authorTaboga, Oscar Alberto
dc.contributor.authorMarti, Hector Ruben
dc.contributor.authorKeurentjes, Joost
dc.contributor.authorSulpice, Ronan
dc.contributor.authorIshihara, Hirofumi
dc.contributor.authorStitt, Mark
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorCarrari, Fernando
dc.date.accessioned2017-09-11T12:55:58Z
dc.date.available2017-09-11T12:55:58Z
dc.date.issued2016
dc.identifier.issn0022-0957 (Print)
dc.identifier.issn1460-2431 (Online)
dc.identifier.otherhttps://doi.org/10.1093/jxb/erw185
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1187
dc.identifier.urihttps://academic.oup.com/jxb/article/67/14/4091/2197644
dc.description.abstractImproving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.eng
dc.formatapplication/pdf
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceJournal of experimental botany 67 (14) : 4091–4103 (July 2016)
dc.subjectFitomejoramiento
dc.subjectPlant Breedingeng
dc.subjectArabidopsis
dc.subjectBiomasa
dc.subjectBiomasseng
dc.subjectLoci de Rasgos Cuantitativos
dc.subjectQuantitative Trait Locieng
dc.titleAllelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInst. de Biotecnología
dc.gic150897
dc.description.filFil: Coluccio Leskow, Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Díaz Zirpolo, José Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Obata, Toshihiro. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Kamenetzky, Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
dc.description.filFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Marti, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.filFil: Keurentjes, Joost. Wageningen University. Laboratory of Genetics; Holanda
dc.description.filFil: Sulpice, Ronan. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Ishihara, Hirofumi. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Stitt, Mark. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania
dc.description.filFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.subtypecientifico


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