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Abstract
The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. [ver mas...]
dc.contributor.authorOgrzewalska, María
dc.contributor.authorNieri-Bastos, Fernanda A.
dc.contributor.authorMarcili, Arlei
dc.contributor.authorNava, Santiago
dc.contributor.authorGonzález-Acuña, Daniel
dc.contributor.authorMuñoz-Leal, Sebastian
dc.contributor.authorRuiz-Arrondo, Ignacio
dc.contributor.authorVenzal, José Manuel
dc.contributor.authorMangold, Atilio Jose
dc.contributor.authorLabruna, Marcelo B.
dc.coverage.spatialArgentina (nation)
dc.coverage.spatialChile (nation)
dc.date.accessioned2017-09-05T15:32:05Z
dc.date.available2017-09-05T15:32:05Z
dc.date.issued2016
dc.identifier.issn1877-959X (Print)
dc.identifier.issn1877-9603 (Online)
dc.identifier.otherhttps://doi.org/10.1016/j.ttbdis.2016.01.003
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1134
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub
dc.description.abstractThe tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.sourceTicks and tick-borne diseases 7 (3) : 439-442. (April 2016)
dc.subjectRickettsia
dc.subjectAmblyomma
dc.subjectZona de Montaña
dc.subjectHighlandseng
dc.subject.otherRegión Patagónica
dc.subject.otherAmblyomma Parvitarsum
dc.titleA novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/acceptedVersion
dc.description.origenEEA Rafaela
dc.gic150470
dc.description.filFil: Ogrzewalska, María. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses; Brasil
dc.description.filFil: Nieri-Bastos, Fernanda A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
dc.description.filFil: Marcili, Arlei. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil. Universidade de Santo Amaro. Medicina Veterinária e Bem estar animal; Brasil
dc.description.filFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: González-Acuña, Daniel. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
dc.description.filFil: Muñoz-Leal, Sebastian. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
dc.description.filFil: Ruiz-Arrondo, Ignacio. Universidad de Zaragoza. Departamento de Patología Animal, Facultad de Veterinaria; España
dc.description.filFil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte. Laboratorio de Vectores y enfermedades transmitidas; Uruguay
dc.description.filFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
dc.subtypecientifico


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