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Abstract
The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was [ver mas...]
dc.contributor.authorRobles, Carlos Alejandro
dc.contributor.authorNielsen, Klaus
dc.contributor.authorGall, David
dc.contributor.authorWillems, Priscila Mabel
dc.date.accessioned2022-02-14T11:44:37Z
dc.date.available2022-02-14T11:44:37Z
dc.date.issued2009
dc.identifier.issn0165-2427
dc.identifier.otherhttps://doi.org/10.1016/j.vetimm.2008.09.007
dc.identifier.urihttp://hdl.handle.net/20.500.12123/11134
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0165242708003486
dc.description.abstractThe live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. The performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting SRB51 antibodies were evaluated. A homogeneous group of twenty-five 10-month-old Hereford heifers was used. The animals were bled on day 0 and then subcutaneously vaccinated with 2 ml of a commercially available SRB51 vaccine (Schering-PloughTM) containing 1 107 to 3.4 107 viable cells. Blood samples without anticoagulant for sera obtaining were then collected at days 30, 90, 210 and 360 post-vaccination. To detect the SRB51 antibodies, Brucella ovis hot saline extract, B. ovis RLPS (RLPS), and SRB51-RLPS were used. The buffered antigen plate agglutination test and an indirect enzyme-linked immunoassay (I-ELISA) using the smooth LPS (SLPS) antigen from B. abortus were used as control tests. All the sera samples were negative in the BPA test and in the standard I-ELISA using the SLPS. The SRB51-RLPS and the B. ovis RLPS antigens performed better than the B. ovis hot saline extract antigen.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Immunology and Immunopathology 127 : 153-155 (2009)es_AR
dc.subjectBrucellaes_AR
dc.subjectBrucella abortuses_AR
dc.subjectBrucellosiseng
dc.subjectBrucelosises_AR
dc.subjectAntígenoses_AR
dc.subjectAntigenseng
dc.subjectAnticuerposes_AR
dc.subjectAntibodieseng
dc.subjectTécnicas Inmunológicases_AR
dc.subjectImmunological Techniqueseng
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subject.otherRegión Patagónicaes_AR
dc.subject.otherInmunoensayoes_AR
dc.titleEvaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heiferses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEstación Experimental Agropecuaria Barilochees_AR
dc.description.filFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentinaes_AR
dc.description.filFil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadáes_AR
dc.description.filFil: Gall, David. Canadian Food Inspection Agency. Ottawa Laboratories; Canadáes_AR
dc.description.filFil: Willems, Priscila Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentinaes_AR
dc.subtypecientifico


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