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Abstract
Bovine babesiosis caused by Babesia bigemina and B. bovis is an economically relevant tick-borne disease distributed over tropical and subtropical world regions. Animals that recover from the clinical disease can remain persistently infected, and those carriers are epidemiologically relevant since they can act as a source of infection to other animals through the tick bite. According to the manual of the World Organisation for Animal Health (OIE), the [ver mas...]
dc.contributor.authorDe La Fourniere, Sofía Ana María
dc.contributor.authorPaoletta, Martina
dc.contributor.authorGuillemi, Eliana Carolina
dc.contributor.authorSarmiento, Nestor Fabian
dc.contributor.authorDonati, Pablo Alejandro
dc.contributor.authorWilkowsky, Silvina Elizabeth
dc.contributor.authorFarber, Marisa Diana
dc.dateinfo:eu-repo/date/embargoEnd/2022-12-10
dc.date.accessioned2021-12-10T14:54:34Z
dc.date.available2021-12-10T14:54:34Z
dc.date.issued2021-08
dc.identifier.issn0304-4017
dc.identifier.otherhttps://doi.org/10.1016/j.vetpar.2021.109493
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10884
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0304401721001527
dc.description.abstractBovine babesiosis caused by Babesia bigemina and B. bovis is an economically relevant tick-borne disease distributed over tropical and subtropical world regions. Animals that recover from the clinical disease can remain persistently infected, and those carriers are epidemiologically relevant since they can act as a source of infection to other animals through the tick bite. According to the manual of the World Organisation for Animal Health (OIE), the recommended molecular diagnosis test for both parasites is a nested polymerase chain reaction (nPCR) based on an amplification of a fragment of the rap-1 gene. Since nPCRs are time consuming, have a higher cost and risk of contamination, we propose a single step PCR for B. bigemina (BbiVESA) and B. bovis (BboVESA) based on the amplification of the multi-copy ves-1α gene. We developed these methods and we achieved a detection limit of 1 × 10−12 % parasitemia for B. bigemina and of 1 × 10−6 % for B. bovis using reference strains, which compared to the reference OIE tests, results in an improvement in sensitivity of six orders for B. bigemina. Finally, we tested 48 field samples from a babesiosis enzootic region where we were able to detect a higher proportion of positive animals with both VESA methods than with the reference rap-1 nPCRs. This difference was statistically significant for each Babesia species. Concordance between both diagnostic schemes based on Cohen's kappa coefficient showed minimal to non-agreement (κ = 0.32) for B. bigemina and non-agreement (κ = 0.16) for B. bovis since BbiVESA and BboVESA PCR tests showed a significantly higher detection capacity. In conclusion, the high sensitivity of the assay, together with the lower demand of time and reagents make the VESA PCR methods developed here a valuable diagnostic tool for the molecular detection and epidemiological survey of both Babesia pathogens.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceVeterinary Parasitology 296 : 109493 (August 2021)es_AR
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectBabesia bigeminaes_AR
dc.subjectBabesia bovises_AR
dc.subjectDiagnósticoes_AR
dc.subjectDiagnosiseng
dc.subjectPCReng
dc.subjectInfecciónes_AR
dc.subjectInfectioneng
dc.titleDevelopment of highly sensitive one step-PCR tests for improved detection of B. bigemina and B. bovises_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: De La Fourniere, Sofía Ana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentinaes_AR
dc.description.filFil: De La Fourniere, Sofía Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentinaes_AR
dc.description.filFil: Paoletta, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentinaes_AR
dc.description.filFil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Donati, Pablo Alejandro. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Anestesiología y Manejo del Dolor; Argentinaes_AR
dc.description.filFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentinaes_AR
dc.description.filFil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentinaes_AR
dc.description.filFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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