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Abstract
One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass [ver mas...]
dc.contributor.authorOntañon, Ornella Mailen
dc.contributor.authorBedő, Soma
dc.contributor.authorGhio, Silvina
dc.contributor.authorGarrido, Mercedes Maria
dc.contributor.authorTopalian, Juliana
dc.contributor.authorJahola, Dóra
dc.contributor.authorFehér, Anikó
dc.contributor.authorValacco, Maria Pia
dc.contributor.authorCampos, Eleonora
dc.contributor.authorFehér, Csaba
dc.date.accessioned2021-11-15T16:19:12Z
dc.date.available2021-11-15T16:19:12Z
dc.date.issued2021-05
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.otherhttps://doi.org/10.1007/s00253-021-11305-y
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10779
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs00253-021-11305-y
dc.description.abstractOne of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceApplied Microbiology and Biotechnology 105 : 4577-4588 (2021)es_AR
dc.subjectCellulomonaseng
dc.subjectLignocelulosaes_AR
dc.subjectLignocelluloseeng
dc.subjectHidrólisis Enzimáticaes_AR
dc.subjectEnzymatic Hydrolysiseng
dc.subjectBiomasaes_AR
dc.subjectBiomasseng
dc.subject.otherXilanasases_AR
dc.subject.otherXylanaseseng
dc.titleOptimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstructiones_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Ontañon, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bedő, Soma. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungríaes_AR
dc.description.filFil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Ghio, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular.es_AR
dc.description.filFil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Jahola, Dóra. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungríaes_AR
dc.description.filFil: Fehér, Anikó. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungríaes_AR
dc.description.filFil: Valacco, Maria Pia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentinaes_AR
dc.description.filFil: Valacco, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Fehér, Csaba. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungríaes_AR
dc.subtypecientifico


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