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Abstract
Lotus tenuis is a perennial forage legume widely scattered in the flooding Pampa region of Argentina. Even when it is considered a salt-tolerant species, the molecular basis underlying this trait still remains unknown. Intracellular compartmentation of Na+ into vacuoles by NHX antiporters is an important and well known strategy to reduce ion toxicity during salt stress. In this study, the subcellular localization of Lotus tenuis NHX1 was determined by the [ver mas...]
dc.contributor.authorAffinito, María Agostina
dc.contributor.authorEspasandin, Fabiana Daniela
dc.contributor.authorDel Vas, Mariana
dc.contributor.authorAlvarez, Mayra Yanet
dc.contributor.authorAndres, Adriana Noemi
dc.contributor.authorSansberro, Pedro Alfonso
dc.contributor.authorDiaz Paleo, Antonio Horacio
dc.date.accessioned2021-11-11T12:27:31Z
dc.date.available2021-11-11T12:27:31Z
dc.date.issued2021-05
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.otherhttps://doi.org/10.1007/s11240-021-02094-3
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10747
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs11240-021-02094-3
dc.description.abstractLotus tenuis is a perennial forage legume widely scattered in the flooding Pampa region of Argentina. Even when it is considered a salt-tolerant species, the molecular basis underlying this trait still remains unknown. Intracellular compartmentation of Na+ into vacuoles by NHX antiporters is an important and well known strategy to reduce ion toxicity during salt stress. In this study, the subcellular localization of Lotus tenuis NHX1 was determined by the transient expression of a LtNHX1-GFP fusion protein in Nicotiana benthamiana agroinfiltrated leaves, confirming that it is a tonoplast-located NHX antiporter. To study LtNHX1 contribution to salinity tolerance, a transgenic Lotus tenuis line was obtained by Agrobacterium-mediated transformation. Overexpression of the transgene was confirmed by PCR and RT-PCR analyses. Compared to wild-type, transgenic plants displayed less sensitivity and grew better under high salt stress, with higher survival rates, shoot length, and dry weight. These results suggest that the activity of the tonoplast-located LtNHX1 antiporter contributes to Lotus tenuis salt tolerance.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNPA-1126072/AR./Desarrollo de cultivares superiores de especies forrajeras para sistemas ganaderos y agricolo-ganaderos de la Argentina.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Cell, Tissue and Organ Culture (PCTOC) 146 : 565–576 (2021)es_AR
dc.subjectLotus tenuises_AR
dc.subjectEstrés Osmóticoes_AR
dc.subjectOsmotic Stresseng
dc.subjectPlantas Transgénicases_AR
dc.subjectTransgenic Plantseng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subject.otherEstrés Salinoes_AR
dc.titleFunctional characterization and intracellular localization of a Lotus tenuis NHX antiporteres_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Affinito, María Agostina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentinaes_AR
dc.description.filFil: Affinito, María Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires; Argentinaes_AR
dc.description.filFil: Affinito, María Agostina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentinaes_AR
dc.description.filFil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.es_AR
dc.description.filFil: Alvarez, Mayra Yanet. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Alvarez, Mayra Yanet. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Andrés, Adriana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Forrajeras; Argentina.es_AR
dc.description.filFil: Andrés, Adriana Noemi. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentinaes_AR
dc.description.filFil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina.es_AR
dc.description.filFil: Diaz Paleo, Antonio Horacio. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Ciencias Agrarias, Naturales y Ambientales; Argentinaes_AR
dc.subtypecientifico


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