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Resumen
Brucella ovis infection is a major cause of epididymitis and infertility in rams, resulting in reproductive failure and significant economic losses worldwide. The goal of this study was to develop a PCR test targeting specific B. ovis genomic sequences. Specific primer pairs were designed targeting 12 of those ORFs. Samples of blood, serum, semen, urine, and preputial wash were collected from experimentally infected rams (n = 9) every other week up to [ver mas...]
dc.contributor.authorXavier, Mariana N.
dc.contributor.authorSilva, Teane M.A.
dc.contributor.authorCosta, Erica A.
dc.contributor.authorPaixao, Tatiane A.
dc.contributor.authorMoustacas, Valeria S.
dc.contributor.authorCarvalho Junior, Custodio A.
dc.contributor.authorSant’Anna, Felipe M.
dc.contributor.authorRobles, Carlos Alejandro
dc.contributor.authorGouveia, Aurora M.G.
dc.contributor.authorLage, Andrey P.
dc.contributor.authorTsolis, Renee M.
dc.contributor.authorSantos, Renato L.
dc.date.accessioned2021-10-19T11:33:58Z
dc.date.available2021-10-19T11:33:58Z
dc.date.issued2010
dc.identifier.issn0378-1135
dc.identifier.otherhttps://doi.org/10.1016/j.vetmic.2010.02.037
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10522
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S037811351000132X
dc.description.abstractBrucella ovis infection is a major cause of epididymitis and infertility in rams, resulting in reproductive failure and significant economic losses worldwide. The goal of this study was to develop a PCR test targeting specific B. ovis genomic sequences. Specific primer pairs were designed targeting 12 of those ORFs. Samples of blood, serum, semen, urine, and preputial wash were collected from experimentally infected rams (n = 9) every other week up to 180 days post infection (dpi), when tissue samples were obtained. Blood, serum, semen, urine, and preputial wash samples were obtained, in weekly intervals for 1 month, from eight rams belonging to a B. ovis-free flock. Semen samples were also obtained from rams belonging to naturally infected flocks (n = 40). The limit of detection of this PCR protocol was 100, 10, and 1 CFU/mL for semen, urine and prepucial wash samples, respectively. Sensitivity and specificity values obtained with this PCR method were similar to that of bacteriology when evaluating biological samples. Agreement between PCR and bacteriology results was greater than 90%. These results clearly indicate that this speciesspecific PCR method is highly efficient for the diagnosis of B. ovis infection in semen, urine, preputial wash and tissue samples from infected rams.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Microbiology 145 (1-2) : 158-164 (Septiembre 2010)es_AR
dc.subjectBrucellaes_AR
dc.subjectBrucella Ovises_AR
dc.subjectEpididimitises_AR
dc.subjectEpididymitiseng
dc.subjectEpididimitis Infecciosa Ovinaes_AR
dc.subjectOvine Infectious Epididymitiseng
dc.subjectPCRes_AR
dc.subjectMorueco
dc.subjectRamseng
dc.subject.otherCarneroses_AR
dc.subject.otherRegión Patagónicaes_AR
dc.titleDevelopment and evaluation of a species-specific PCR assay for the detection of Brucella ovis infection in ramses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEstación Experimental Agropecuaria Barilochees_AR
dc.description.filFil: Xavier, Mariana N. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Silva, Teane M.A. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Costa, Erica A. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Paixao, Tatiane A. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Moustacas, Valeria S. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Carvalho Junior, Custodio A. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Sant’Anna, Felipe M. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.description.filFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentinaes_AR
dc.description.filFil: Gouveia, Aurora M.G. Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinaria Preventiva; Brasiles_AR
dc.description.filFil: Lage, Andrey P. Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinaria Preventiva; Brasiles_AR
dc.description.filFil: Tsolis, Renee M. University of California. Department of Medical Microbiology and Immunology; Estados Unidoses_AR
dc.description.filFil: Santos, Renato L. Universidade Federal de Minas. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária; Brasiles_AR
dc.subtypecientifico


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