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Abstract
To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, [ver mas...]
dc.contributor.authorZanetti, Flavia Adriana
dc.contributor.authorCardona, Romina
dc.contributor.authorFederico, Carlos Rodolfo
dc.contributor.authorChimeno Zoth, Silvina Andrea
dc.contributor.authorCalamante, Gabriela
dc.date.accessioned2017-08-28T13:20:59Z
dc.date.available2017-08-28T13:20:59Z
dc.date.issued2016
dc.identifier.issn1995-820X (Online)
dc.identifier.issn1674-0769 (Print)
dc.identifier.otherhttps://doi.org/10.1007/s12250-015-3680-6
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1051
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs12250-015-3680-6
dc.description.abstractTo study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVirologica sinica 31(3) : 266–269. (June 2016)eng
dc.subjectEnfermedades de los Animales
dc.subjectPollo
dc.subjectChickenseng
dc.subjectAnimal Diseaseseng
dc.subjectAnimal Viruseseng
dc.subjectVirus de los Animales
dc.subject.otherCanarypox
dc.titleRecombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickenseng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.origenInst. de Biotecnología
dc.gic152257
dc.description.filFil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
dc.description.filFil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
dc.description.filFil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina
dc.description.filFil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.subtypecientifico


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