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Abstract
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 [ver mas...]
dc.contributor.authorRobert, German
dc.contributor.authorMuñoz, Nacira Belen
dc.contributor.authorMelchiorre, Mariana
dc.contributor.authorSanchez, Federico
dc.contributor.authorLascano, Hernan Ramiro
dc.date.accessioned2017-08-25T16:48:20Z
dc.date.available2017-08-25T16:48:20Z
dc.date.issued2014-07-27
dc.identifier.citation:Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.0101747es_AR
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0101747
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1045
dc.identifier.urihttp://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable
dc.description.abstractThe mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.subjectGlycine Maxes_AR
dc.subjectSoja
dc.subjectSoybeanseng
dc.subjectBradyrhizobium Japonicum
dc.subjectEstrés Osmótico
dc.subjectOsmotic Stresseng
dc.subjectGenes
dc.subject.otherAnti-Apoptotic Gene Ced-9eng
dc.subject.otherEstrés Salino
dc.subject.otherSaline Stresseng
dc.titleExpression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formationes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.filFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
dc.description.filFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
dc.description.filFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
dc.description.filFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
dc.description.filFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
dc.subtypecientifico


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