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Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation

Abstract
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 [ver mas...]
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions. [Cerrar]
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Author
Robert, German;   Muñoz, Nacira Belen;   Melchiorre, Mariana;   Sanchez, Federico;   Lascano, Hernan Ramiro;  
Date
2014-07-27
ISSN
1932-6203
URI
http://hdl.handle.net/20.500.12123/1045
http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable
DOI
https://doi.org/10.1371/journal.pone.0101747
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Tipo de documento
artículo
Palabras Claves
Glycine Max; Soja; Soybeans; Bradyrhizobium Japonicum; Estrés Osmótico; Osmotic Stress; Genes; Anti-Apoptotic Gene Ced-9; Estrés Salino; Saline Stress;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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