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Abstract
The pathogen enterohemorrhagic Escherichia coli (EHEC) O157: H7 is responsible for hemorrhagic colitis and hemolytic uremic syndrome in humans [1]. During the colonization process in the gastrointestinal tract, EHEC needs to adapt to changes in nutrient availability [2]. The objective of this study was to evaluate the influence of glucose on physiology and processes involved in the pathogenesis of EHEC O157: H7 in order to improve our understanding of [ver mas...]
dc.contributor.authorMarques Da Silva, Wanderson
dc.contributor.authorTaibo, Catalina Beatriz
dc.contributor.authorSabio Y Garcia, Julia Veronica
dc.contributor.authorLarzabal, Mariano
dc.contributor.authorCataldi, Angel Adrian
dc.date.accessioned2021-09-10T12:12:36Z
dc.date.available2021-09-10T12:12:36Z
dc.date.issued2020-04
dc.identifier.issn1431-9276
dc.identifier.otherhttps://doi.org/10.1017/S1431927620001129
dc.identifier.urihttp://hdl.handle.net/20.500.12123/10224
dc.identifier.urihttps://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/role-of-glucose-in-the-pathology-of-ehec-o157-h7/4F5327F46D17EE9933438979E0134B28
dc.description.abstractThe pathogen enterohemorrhagic Escherichia coli (EHEC) O157: H7 is responsible for hemorrhagic colitis and hemolytic uremic syndrome in humans [1]. During the colonization process in the gastrointestinal tract, EHEC needs to adapt to changes in nutrient availability [2]. The objective of this study was to evaluate the influence of glucose on physiology and processes involved in the pathogenesis of EHEC O157: H7 in order to improve our understanding of the mechanisms controlling EHEC growth and survival in the bovine gut. In this study we first analyzed the growth rate of EHEC O157: H7 Rafaela II clade 8, a strain isolated from a bovine in Argentina, grown in the medium DMEM supplemented with either 4.5% glucose (Highglucose - DHG) or 1% glucose (Low-glucose - DLG). In addition, we assessed the bacterial adhesion capacity and actin pedestal formation induced by EHEC [3] by performing infection assays. For this purpose, Caco-2 epithelial cells were exposed for 5 h with Rafaela II grown with the different concentrations of glucose. Subsequently, the samples were fixed (paraformaldehyde 4%) and permeabilized (triton); actin and nucleic acids (DNA) were stained with rhodamine-phalloidin and TOPRO-3, respectively. Bacterial adhesion capacity and pedestal formation of cells were evaluated using a Leica TCS SP5 laser scanning confocal microscope (MC). Each Image was acquired by monitoring a single focal plane over time (xyt scanning mode) using a 40X/1.25 oil objective lens and 543nm HeNe and 633 nm HeNe lasers. The frequency and resolution for acquiring images were set at 200 Hz and 1,024 x 1,024 pixels, while maintaining the same settings for laser powers, gain, and offset.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherCambridge University Presses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceMicroscopy and Microanalysis 26 (Suppl 1) : 181-182 (Abril 2020)es_AR
dc.subjectGlucoseeng
dc.subjectGlucosaes_AR
dc.subjectPathogenseng
dc.subjectOrganismos Patógenoses_AR
dc.subjectEscherichia colies_AR
dc.subjectPathogenesiseng
dc.subjectPatogénesises_AR
dc.titleThe role of glucose in the pathology of EHEC O157: H7es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentinaes_AR
dc.description.filFil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Sabio Y Garcia, Julia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentinaes_AR
dc.description.filFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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