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The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection

Abstract
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int [ver mas...]
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. [Cerrar]
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Author
Rabinovitz, Bettina Carol;   Larzabal, Mariano;   Vilte, Daniel Alejandro;   Cataldi, Angel Adrian;   Mercado, Elsa Cristina;  
Fuente
Vaccine 34 (25) : 2793-2797. (May 2016)
Date
2016-05
ISSN
0264-410X
URI
http://hdl.handle.net/20.500.12123/1001
http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109
DOI
https://doi.org/10.1016/j.vaccine.2016.04.056
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Escherichia coli; Vaccination; Vacunación; Mice; Ratón; Infection; Infección;
Derechos de acceso
Restringido
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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