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Mostrando ítems 1-11 de 11
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Aplicaciones y perspectivas de la edición génica de plantas mediante CRISPR-Cas9 = Applications and perspectives of plant gene editing using CRISPR-Cas9
(Universidad de Morón, 2022)Los avances en materia de genética y biotecnología, introducen una dimensión novedosa en la utilización de herramientas de edición génica en plantas. En el presente trabajo se relevaron 164 publicaciones científicas que ... -
Baculovirus transduction in mammalian cells is affected by the production of type I and III interferons, which is mediated mainly by the cGAS-STING pathway
(American Society for Microbiology, 2020-11)The baculovirus Autographa californica multiple nucleopolyhedrovirus is an insect virus with a circular double-stranded DNA genome, which, among other multiple biotechnological applications, is used as an expression vector ... -
Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato
(Keystone Symposia on Molecular and Cellular Biology, 2021-03)StPPO2 gene editing was analyzed by Agrobacterium mediated transformation with CR-PPO vector, ribonucleoprotein complexes (RNP-PPO) transfection to protoplasts, and CR-PPO transient expression in protoplasts, yielding ... -
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
(Springer Netherlands, 2021-01-25)Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ... -
Edición génica con el sistema CRISPR/Cas9: historia de su descubrimiento y alcances en agricultura
(Universidad de Los Andes, Venezuela, 2021-04)La conversión del sistema inmune procariota CRISPR/Cas9 en una herramienta molecular para edición génica representa el avance tecnológico más destacado de la última década, y ha revolucionado tanto la investigación básica ... -
Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm
(Frontiers Media, 2021-07)Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ... -
Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa
(Frontiers Media, 2022-04)Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research ... -
Multiple challenges in the development of commercial crops using CRISPR technology
(Elsevier, 2023-10)The CRISPR/Cas system is a highly efficient and versatile tool for editing plant genomes, with the potential to accelerate breeding programs and improve the sustainability of food production. Nevertheless, technical ... -
Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing
(Springer, 2022-07)The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ... -
State of the art of genetic engineering in potato: from the first report to its future potential
(Frontiers Media, 2022-01)Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, ... -
The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa
(Springer, 2022-12)The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ...