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Nitrate (NO−3) supplementation is an effective methane (CH4) mitigation strategy for ruminants but may produce nitrite (NO−2) toxicity. It has been reported that rumen protozoa have greater ability for NO−3 and NO−2 reduction than bacteria. It was hypothesised
that the absence of ruminal protozoa in sheep may lead to higher NO−2 accumulation in the rumen and a higher blood methaemoglobin (MetHb) concentration.
An in vivo experiment was conducted with
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dc.contributor.author | Villar, Maria Laura | |
dc.contributor.author | Hegarty, Roger Stephen | |
dc.contributor.author | Clay, Jonathon William | |
dc.contributor.author | Smith, Katherine Anne | |
dc.contributor.author | Godwin, Ian Robert | |
dc.contributor.author | Nolan, John Vivian | |
dc.date.accessioned | 2020-06-16T19:06:21Z | |
dc.date.available | 2020-06-16T19:06:21Z | |
dc.date.issued | 2020-04 | |
dc.identifier.issn | 1439-0396 | |
dc.identifier.other | https://doi.org/10.1111/jpn.13365 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/7421 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1111/jpn.13365 | |
dc.description.abstract | Nitrate (NO−3) supplementation is an effective methane (CH4) mitigation strategy for ruminants but may produce nitrite (NO−2) toxicity. It has been reported that rumen protozoa have greater ability for NO−3 and NO−2 reduction than bacteria. It was hypothesised that the absence of ruminal protozoa in sheep may lead to higher NO−2 accumulation in the rumen and a higher blood methaemoglobin (MetHb) concentration. An in vivo experiment was conducted with defaunated (DEF) and faunated (FAU) sheep supplemented with 1.8% NO−3 in DM. The effects of rumen protozoa on concentrations of plasma and ruminal NO−3 and NO−2, blood MetHb, ruminal volatile fatty acid (VFA) and ruminal ammonia (NH3) were investigated. Subsequently, two in vitro experiments were conducted to determine the contribution of protozoa to NO−3 and NO−2 reduction rates in DEF and FAU whole rumen digesta (WRD) and its liquid (LIQ) and solid (SOL) fractions, incubated alone (CON), with the addition of NO−3 or with the addition of NO−2. The results from the in vivo experiment showed no differences in total VFA concentrations, although ruminal NH3 was greater (p < .01) in FAU sheep. Ruminal NO−3, NO−2 and plasma NO−2 concentrations tended to increase (p < .10) 1.5 hr after feeding in FAU relative to DEF sheep. In vitro results showed that NO−3 reduction to NH3 was stimulated (p < .01) by incoming NO−3 in both DEF and FAU relative to CON digesta. However, adding NO−3 increased (p < .05) the rate of NO−2 accumulation in the SOL fraction of DEF relative to both fractions of FAU digesta. Results observed in vivo and in vitro suggest that NO−3 and NO−2 are more rapidly metabolised in the presence of rumen protozoa. Defaunated sheep may have an increased risk of NO−2 poisoning due to NO−2 accumulation in the rumen. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Wiley | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Journal of Animal Physiology and Animal Nutrition’s : 1-14 (Abril 2020) | es_AR |
dc.subject | Rumiante | es_AR |
dc.subject | Ruminants | eng |
dc.subject | Rumen | es_AR |
dc.subject | Nitrito Reductasa | es_AR |
dc.subject | Nitrito Reductase | eng |
dc.subject | Digestión Ruminal | es_AR |
dc.subject | Rumen Digestion | eng |
dc.subject | Oveja | es_AR |
dc.subject | Ewes | eng |
dc.title | Dietary nitrate and presence of protozoa increase nitrate and nitrite reduction in the rumen of sheep | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Estación Experimental Agropecuaria Bariloche | es_AR |
dc.description.fil | Fil: Villar, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina | es_AR |
dc.description.fil | Fil: Hegarty, Roger Stephen. University of New England. School of Environmental and Rural Science; Australia | es_AR |
dc.description.fil | Fil: Clay, Jonathon William. University of New England. School of Science and Technology; Australia | es_AR |
dc.description.fil | Fil: Smith, Katherine Anne. University of New England. School of Environmental and Rural Science; Australia | es_AR |
dc.description.fil | Fil: Godwin, Ian Robert. University of New England. School of Environmental and Rural Science; Australia | es_AR |
dc.description.fil | Fil: Nolan, John Vivian. University of New England. School of Environmental and Rural Science; Australia | es_AR |
dc.subtype | cientifico |
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