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Mostrando ítems 1-20 de 31
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A synthesis of functional contributions of rhizobacteria to growth promotion in diverse crops
Brambilla, Silvina Maricel; Stritzler, Margarita; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-12)The endophytic microbes are known to live asymptomatically inside their host and play crucial roles in the growth, development, fitness, and diversification of plants. The plant-endophyte association ranges from mutualism ... -
Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Soto, Gabriela Cinthia; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Springer, 2018-08)As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand ... -
Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
Garcia, Araceli Nora; Ayub, Nicolás Daniel; Fox, Ana Romina; Gomez, Maria Cristina; Dieguez, Maria Jose; Pagano, Elba Maria; Berini, Carolina Andrea; Muschietti, Jorge Prometeo; Soto, Gabriela Cinthia (BMC Plant Biology, 2014-09-17)Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. ... -
CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ... -
Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
Setten, Lorena; Soto, Gabriela Cinthia; Mozzicafreddo, Matteo; Fox, Ana Romina; Lisi, Christian Daniel; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Diaz Paleo, Antonio Horacio; Ayub, Nicolás Daniel (2013-05-13)Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the ... -
Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases
Pascuan, Cecilia Gabriela; Fox, Ana Romina; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (Springer, 2015-10)The vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix ... -
Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
Soto, Gabriela Cynthia; Fox, Ana Romina; Ayub, Nicolás Daniel (Springer, 2013-08)Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially ... -
Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island
Iribarren, Maria Josefina; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Oxford University Press, 2015-11)Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response ... -
Genetic diversity in a world germplasm collection of tall fescue
Cuyeu, Alba Romina; Rosso, Beatriz Susana; Pagano, Elba Maria; Soto, Gabriela; Fox, Ana Romina; Ayub, Nicolás Daniel (2013)Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated ... -
The genetic diversity of wild rescuegrass is associated with precipitation levels
Cuyeu, Alba Romina; Pagano, Elba Maria; Rosso, Beatriz Susana; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (Springer, 2014-04-27)The genus Bromus belongs to the family Poaceae, contains over 160 annual and perennial species of grasses, varying in ploidy level from diploid (2n = 14) to dodecaploid (2n = 70) (Fortune et al. 2008). One of the most ... -
High-quality forage production under salinity by using a salt-tolerant AtNXH1-expressing transgenic alfalfa combined with a natural stress-resistant nitrogen-fixing bacterium
Stritzler, Margarita; Pagano, Elba Maria; Berini, Carolina Andrea; Gomez, Maria Cristina; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (Elsevier, 2018-06)Alfalfa, usually known as the “Queen of Forages”, is the main source of vegetable protein to meat and milk production systems worldwide. This legume is extremely rich in proteins due to its highly efficient symbiotic ... -
LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
Moriconi, Victoria; Sellaro, Romina Vanesa; Ayub, Nicolás Daniel; Soto, Gabriela; Rugnone, Matias Leandro; Shah, Rashmi; Pathak, Gopal P.; Gärtner, Wolfgang; Casal, Jorge José (Wiley, 2013-10)In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst ... -
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940
Fox, Ana Romina; Soto, Gabriela Cinthia; Valverde, Claudio Fabián; Russo, Daniela Marta; Lagares, Antonio; Zorreguieta, Ángeles; Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Mercado Blanco, Jesús; Dixon, Ray; Ayub, Nicolás Daniel; Alleva, Karina Edith (2016)A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas ... -
Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions
Jozefkowicz, Cintia; Frare, Romina Alejandra; Odorizzi, Ariel; Arolfo, Valeria; Pagano, Elba Maria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (2018-05)Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic ... -
Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana Laura; Piccinetti, Carlos Fabian; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-08)Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the ... -
Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia; Jozefkowicz, Cintia (2016)Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the ... -
mRNA biogenesis-related helicase eIF4AIII from Arabidopsis thaliana is an important factor for abiotic stress adaptation
Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Alleva, Karina Edith; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (2016)Similar to other plant species, Arabidopsis has a huge repertoire of predicted helicases, including the eIF4AIII factor, a putative component of the exon junction complex related to mRNA biogenesis. In this article, we ... -
pBAR–H3.2, a native-optimized binary vector to bypass transgene silencing in alfalfa
Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Kapros, Tamas; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2020-03)Alfalfa is the main forage worldwide due to its high biomass production, excellent nutritional qualities and adaptation to a wide range of environments (Singer et al. 2018). Besides, due to its ability to grow without ... -
Pip water transport and its pH dependence are regulated by tetramer stoichiometry
Jozefkowicz, Cintia; Sigaut, Lorena; Scochera, Florencia; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel; Pietrasanta, Lía Isabel; Amodeo, Gabriela; Gonzalez Flecha, Francisco Luis; Alleva, Karina Edith (2016)Many plasma membrane channels form oligomeric assemblies, and heterooligomerization has been described as a distinctive feature of some protein families. In the particular case of plant plasma membrane aquaporins (PIPs), ... -
Plant growth-promoting bacterium Pseudomonas fluorescens FR1 secrets a novel type of extracellular polyhydroxybutyrate polymerase involved in abiotic stress response in plants
Stritzler, Margarita; Diez Tissera, Ana; Soto, Gabriela; Ayub, Nicolás Daniel (Springer, 2018)Objectives Identification of novel microbial factors contributing to plant protection against abiotic stress. Results The genome of plant growth-promoting bacterium Pseudomonas fluorescens FR1 contains a short mobile ...