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Mostrando ítems 1-12 de 12
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25S–18S rDNA IGS of Capsicum: molecular structure and comparison
Grabiele, Mauro; Debat, Humberto Julio; Moscone, Eduardo Alberto; Ducasse, Daniel Adrian (Springer, 2012-02)The primary and secondary structures of the intergenic spacer (IGS) between the 3′-end of 25S ribosomal RNA (rRNA) gene and the 5′-end of 18S rRNA gene are described for the cultivated chili pepper Capsicum pubescens. The ... -
An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
Giachero, María Lorena; Marquez, Nathalie; Gallou, Adrien; Luna, Celina Mercedes; Declerck, Stéphane; Ducasse, Daniel Adrian (2017-06)In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected ... -
The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.)
Debat, Humberto Julio; Grabiele, Mauro; Aguilera, Patricia Mabel; Bubillo, Rosana Elizabeth; Zapata, Pedro Darío; Marti, Dardo Andrea; Ducasse, Daniel Adrian (2014-10)We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, ... -
Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
Scaldaferro, Marisel Analía; Grabiele, Mauro; Seijo, Jose Guillermo; Debat, Humberto Julio; Romero, María Victoria; Ducasse, Daniel Adrian; Prina, Alberto Raul; Moscone, Eduardo Alberto (2014)Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum ... -
Exploring the genes of Yerba Mate (Ilex paraguariensis A. St.-Hil.) by NGS and De Novo Transcriptome Assembly
Debat, Humberto Julio; Grabiele, Mauro; Aguilera, Patricia Mabel; Bubillo, Rosana Elizabeth; Otegui, Mónica B.; Ducasse, Daniel Adrian; Zapata, Pedro Darío; Marti, Dardo Andrea (2014-10-16)Yerba mate (Ilex paraguariensis A. St.-Hil.) is an important subtropical tree crop cultivated on 326,000 ha in Argentina, Brazil and Paraguay, with a total yield production of more than 1,000,000 t. Yerba mate presents a ... -
Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization
Grabiele, Mauro; Debat, Humberto Julio; Scaldaferro, Marisel Analía; Aguilera, Patricia Mabel; Moscone, Eduardo Alberto; Seijo, José Guillermo; Ducasse, Daniel Adrian (Elsevier, 2018-08)Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin ... -
Occurrence of the complete cycle of Puccinia sorghi Schw. in Argentina and implications on the common corn rust epidemiology
Guerra, Fernando Andres; de Rossi, Roberto Luis; Brücher, Elsa; Vuletic, Ezequiel Esteban; Plazas, Maria Cristina; Guerra, Gustavo Dario; Ducasse, Daniel Adrian (Springer, 2018-12)The life-cycle of Puccinia sorghi, a heteroecious fungus, consists of five well-defined spore stages. The uredinial and telial stages are completed on the primary host (maize) whereas spermagonial and aecial stages occur ... -
Plant microRNAs: Recent Advances and Future Challenges
Debat, Humberto Julio; Ducasse, Daniel Adrian (2014-12)MicroRNAs (miRNAs) are small ∼20–24 nt species of non-coding RNAs that modulate plant gene expression by means of gene silencing through sequence-specific inhibition of target mRNAs. MiRNAs derive from pol-II transcription ... -
Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
Dal Zotto, Angelica; Balzarini, Mónica Graciela; Raigon, Juan Manuel; Rossini, Mirta Noemi; Ducasse, Daniel Adrian (Wiley, 2014-01)A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus ... -
A strain of Bacillus subtilis subsp. subtilis shows a specific antagonistic activity against the soil-borne pathogen of onion Setophoma terrestris
Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrian (2015)Pink Root is among the major diseases of onion and its causal agent, Setophoma terrestris, is one of the most severe pathogens in soils of tropical and subtropical climates. The management of Pink Root is difficult and the ... -
Switching between monocot and dicot crops in rotation schemes of Argentinean productive fields results in an increment of arbuscular mycorrhizal fungi diversity
Albarracín Orio, Andrea Georgina; Brücher, Elsa; Ducasse, Daniel Adrian (2016-02)Despite the importance of mycorrhizal symbiosis, we understand little how different soil managements affect arbuscular mycorrhizal fungi (AMF) communities. Crop rotation is recommended in sustainable agriculture because ... -
Use of silencing reporter and agroinfiltration transient assays to evaluate the potential of hpRNA construct to induce multiple tospovirus resistance
Debat, Humberto Julio; Grabiele, Mauro; Ducasse, Daniel Adrian; Lopez Lambertini, Paola Maria (2015-12)Tospoviruses are devastating plant viruses causing severe economic losses in a diverse range of crops worldwide. Here, we describe the development and evaluation of an RNA interference (RNAi) broad-spectrum virus resistance ...