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Mostrando ítems 1-19 de 19
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Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
Pujato, Silvina A.; Galliani, Valentina; Irazoqui, Jose Matias; Amadio, Ariel; Quiberoni, Andrea; Mercanti, Diego (Elsevier, 2021-07)A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They ... -
Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis
Kurth, Daniel German; Amadio, Ariel F.; Ordoñez, Omar F.; Allbarracín, Virginia H.; Farías, María E.; Gärtner, Wolfgang (2017)Modern stromatolites thrive only in selected locations in the world. Socompa Lake, located in the Andean plateau at 3570 masl, is one of the numerous extreme Andean microbial ecosystems described over recent years. Extreme ... -
Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
Abatángelo, Virginia; Peressutti Bacci, Natalia; Boncompain, Carina A.; Amadio, Ariel; Carrasco, Soledad; Suárez, Cristian A.; Morbidoni, Héctor R. (Plos One, 2017-07)Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display ... -
Characterization of novel proteases identified by metagenomic analysis from dairy stabilization ponds
Irazoqui, Jose Matias; Eberhardt, María Florencia; Santiago, Gonzalo Manuel; Amadio, Ariel (Springer, 2023-05)Cheese whey is the main by-product of dairy industries. It is used as a raw material for other value-added products, like whey protein concentrate. By using enzymes, this product can be further treated to obtain new higher ... -
Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
Benintende, Graciela Beatriz; Berretta, Marcelo Facundo; Zandomeni, Ruben; Sauka, Diego Hernan; Amadio, Ariel F.; Ortiz, Elio Maximiliano; Navas, Laura Emilce (2017)We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously ... -
Cost-Effective Method to Perform SARS-CoV-2 Variant Surveillance: Detection of Alpha, Gamma, Lambda, Delta, Epsilon, and Zeta in Argentina
Torres, Carolina; Mojsiejczuk, Laura; Acuña, Dolores; Alexay, Sofía; Amadio, Ariel; Aulicino, Paula; Debat, Humberto Julio; Fay, Fabián; Fernandez, Franco Daniel; Giri, Adriana A.; Goya, Stephanie; Konig, Guido Alberto; Lucero, Horacio; Nabaes Jodar, Mercedes Soledad; Pianciola, Luis; Sfalcin, Javier A.; Acevedo, Raúl Maximiliano; Bengoa Luoni, Sofía Ailin; Bolatti, Elisa M.; Brusés, Bettina; Cacciabue, Marco Polo Domingo; Casal, Pablo E.; Cerri, Agustina; Chouhy, Diego; Dus Santos, Maria Jose; Eberhardt, María Florencia; Fernandez, Ailen; Fernandez, Paula Del Carmen; Fernández Do Porto, Darío; Formichelli, Laura; Gismondi, María Ines; Irazoqui, Jose Matias; Lorenzini Campos, Melina; Lusso, Silvina; Marquez, Nathalie; Muñoz Hidalgo, Marianne Graziel; Mussin, Javier; Natale, Mónica; Oria, Griselda; Pisano, María Belén; Posner, Victoria; Puebla, Andrea Fabiana; Viegas, Mariana (Frontiers Media, 2021-12-10)The emergence of SARS-CoV-2 variants with concerning characteristics to public health has attracted the attention of the scientific community and governments both regionally and globally since the end of 2020. The most ... -
Design and evaluation of synthetic bacterial consortia for optimized phenanthrene degradation through the integration of genomics and shotgun proteomics
Macchi, Marianela; Festa, Sabrina; Nieto, Esteban; Irazoqui, Jose Matias; Vega-Vela, Nelson E.; Junca, Howard; Valacco, María Pía; Amadio, Ariel; Morelli, Irma S.; Coppotelli, Bibiana M. (Elsevier, 2021-03)Two synthetic bacterial consortia (SC) composed of bacterial strains Sphingobium sp. (AM), Klebsiella aerogenes (B), Pseudomonas sp. (Bc-h and T), Burkholderia sp. (Bk) and Inquilinus limosus (Inq) isolated from a natural ... -
Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina = Detección por PCR multiplex de especies de Mycoplasma asociadas a ganado lechero en Argentina
Neder, Veronica Elizabeth; Amadio, Ariel; Calvinho, Luis Fernando (Elsevier, 2021)There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex ... -
Dissecting light sensing and metabolic pathways on the millimeter scale in high-altitude modern stromatolites
Alonso-Reyes, Daniel Gonzalo; Galván, Fátima Silvina; Irazoqui, Jose Matias; Amadio, Ariel; Tschoeke, Diogo; Thompson, Fabiano; Albarracín, Virginia Helena; Farias, María Eugenia (Springer, 2022-10)Modern non-lithifying stromatolites on the shore of the volcanic lake Socompa (SST) in the Puna are affected by several extreme conditions. The present study assesses for the first time light utilization and functional ... -
Elucidating the Connection Between the Health-Promoting Properties of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides
Ale, Elisa C.; Ale, Analia; Peralta, Guillermo H.; Irazoqui, Jose Matias; Correa Olivar, Gabriela; Allende Roldán, Victoria; Vinderola, Celso Gabriel; Amadio, Ariel; Bergamini, Carina Viviana; Cazenave, Jimena; Binetti, Ana (MDPI, 2025-02)The potential probiotic properties of Limosilactobacillus fermentum Lf2, an exopolysaccharide (EPS)-producing strain, were assessed in C57BL/6 mice. The aim of this work was to elucidate if these properties could be ... -
First report of Mycoplasma leachii isolation associated with disease in dairy calves in Argentina = Primer aislamiento de Mycoplasma leachii asociado a enfermedad en terneros de tambo en Argentina
Neder, Veronica Elizabeth; Allassia, Martín; Amadio, Ariel; Calvinho, Luis Fernando (2018)There are few reports about the isolation of Mycoplasma species associated with cattle disease in Argentina. In this work we describe the detection of Mycoplasma leachii associated with disease in dairy calves in Santa Fe ... -
Genome-wide association study for somatic cell score in Argentinean dairy cattle
Nani, Juan Pablo; Raschia, Maria Agustina; Poli, Mario Andres; Calvinho, Luis Fernando; Amadio, Ariel (2015-05)This study aimed to understand the genomic architecture of Argentinean dairy herds by measuring linkage disequilibrium (LD) and identifying loci associated with parameters calculated from somatic cell count (SCC). Phenotypic ... -
Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle
Amadio, Ariel; Bono, James L.; Irazoqui, Jose Matias; Larzabal, Mariano; Marques Da Silva, Wanderson; Eberhardt, María Florencia; Riviere, Nahuel Agustin; Gally, David; Manning, Shannon D.; Cataldi, Angel Adrian (Plos, 2021-10)Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina ... -
Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics
Irazoqui, Jose Matias; Eberhardt, María Florencia; Adjad, Maria Mercedes; Amadio, Ariel (PeerJ Publishing, 2022-03-14)Wastewater stabilization ponds are a natural form of wastewater treatment. Their low operation and maintenance costs have made them popular, especially in developing countries. In these systems, effluents are retained for ... -
Omicron Waves in Argentina: Dynamics of SARS-CoV-2 Lineages BA.1, BA.2 and the Emerging BA.2.12.1 and BA.4/BA.5
Torres, Carolina; Nabaes Jodar, Mercedes Soledad; Acuña, Dolores; Zambrana Montaño, Romina Micaela; Culasso, Andrés Carlos Alberto; Amadio, Ariel; Aulicino, Paula; Ceballos, Santiago; Cacciabue, Marco Polo Domingo; Debat, Humberto Julio; Dus Santos, Maria Jose; Eberhardt, María Florencia; Espul, Carlos; Fay, Fabián; Fernández, María Ailén; Fernandez, Franco Daniel; Fernandez Muñoz, Juan Manuel; Ferrini, Florencia; Gallego, Fernando; Giri, Adriana Angélica; Cerri, Agustina; Bolatti, Elisa; Gismondi, Maria Ines; Goya, Stephanie; Gramundi, Iván; Irazoqui, Jose Matias; Konig, Guido Alberto; Leiva, Viviana; Lucero, Horacio; Marquez, Nathalie; Nardi, Cristina; Ortiz, Belén; Pianciola, Luis; Pintos, Carolina Beatriz; Puebla, Andrea Fabiana; Rastellini, Carolina Victoria; Rojas, Alejandro Ezequiel; Sfalcin, Javier; Suárez, Ariel; Tittarelli, Estefanía; Toro, Rosana; Villanova, Gabriela Vanina; Ziehm, María Cecilia; Zimmermann, María Carla; Zunino, Sebastián; Proyecto PAIS Working Group; Valinotto, Laura; Viegas, Mariana (MDPI, 2023-01-22)The COVID-19 pandemic has lately been driven by Omicron. This work aimed to study the dynamics of SARS-CoV-2 Omicron lineages during the third and fourth waves of COVID-19 in Argentina. Molecular surveillance was performed ... -
Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model
Ale, Elisa C.; Irazoqui, Jose Matias; Ale, Analia; Peralta, Guillermo H.; Puntillo, Melisa; Burns, Patricia Graciela; Correa Olivar, Gabriela; Cazenave, Jimena; Bergamini, Carina Viviana; Amadio, Ariel; Binetti, Ana (MDPI, 2024-01)Limosilactobacillus fermentum Lf2 (Lf2) is an autochthonous strain that produces high levels of exopolysaccharides (EPS). The objective of this work was to evaluate the probiotic potential of Lf2 and its relationship with ... -
Single nucleotide polymorphisms in candidate genes and their relation with somatic cell scores in Argentinean dairy cattle
Nani, Juan Pablo; Raschia, Maria Agustina; Carignano, Hugo; Poli, Mario Andres; Calvinho, Luis Fernando; Amadio, Ariel (2015-11)The prevention and control of bovine mastitis by enhancing natural defenses in animals is important to improve the quality of dairy products. Mastitis resistance is a complex trait which depends on genetic components, as ... -
The Lambda Variant in Argentina: Analyzing the Evolution and Spread of SARS-CoV-2 Lineage C.37
Nabaes Jodar, Mercedes Soledad; Torres, Carolina; Mojsiejczuk, Laura; Acuña, Dolores; Valinotto, Laura Elena; Goya, Stephanie; Natale, Monica; Lusso, Silvina; Alexay, Sofia; Amadio, Ariel; Irazoqui, Jose Matias; Fernandez, Franco Daniel; Acevedo, Maria Elina; Alvarez Lopez, Cristina; Angelletti, Andres; Aulicino, Paula; Bolatti, Elisa; Brusés, Bettina; Cacciabue, Marco Polo Domingo; Cavatorta, Ana; Cerri, Agustina; Cordero, Andres; Debat, Humberto Julio; Dus Santos, Maria Jose; Eberhardt, María Florencia; Ercole, Regina; Espul, Carlos; Farber, Marisa Diana; Fay, Fabián; Fernandez, Ailen; Ferrini, Florencia; Formichelli, Laura; Ceballos, Santiago; Gallego, Fernando; Giri, Adriana; Gismondi, Maria Ines; Acevedo, Raúl Maximiliano; Gramundi, Ivan; Ibañez, María Eugenia; Konig, Guido Alberto; Leiva, Viviana; Lorenzini Campos, Melina; Lucero, Horacio; Marquez, Nathalie; Mazzeo, Melina; Mistchenko, Alicia Susana; Montoto, Luciana; Muñoz Hidalgo, Marianne Graziel; Nadalich, Victoria; Nardi, Cristina; Ortiz, Belén; Pianciola, Luis; Pintos, Carolina; Puebla, Andrea Fabiana; Rastellini, Carolina; Rojas, Alejandro Ezequiel; Sfalcin, Javier; Suarez, Ariel; Theaux, Clara; Thomas, Guillermo; Tittarelli, Estefania; Toro, Rosana; Villanova, Vanina; Wenk, Gretel; Ziehm, Cecilia; Zimmermann, Maria Carla; Zunino, Sebastian; Proyecto PAIS; Viegas, Mariana (MDPI, 2023-06-16)The second wave of COVID-19 occurred in South America in early 2021 and was mainly driven by Gamma and Lambda variants. In this study, we aimed to describe the emergence and local genomic diversity of the SARS-CoV-2 Lambda ... -
β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization
Eberhardt, María Florencia; Irazoqui, Jose Matias; Amadio, Ariel (MDPI, 2021)Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume ...