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A beginner’s guide for FMDV quasispecies analysis: sub-consensus variant detection and haplotype reconstruction using next-generation sequencing
(Oxford Academic Press, 2020-09)Deep sequencing of viral genomes is a powerful tool to study RNA virus complexity. However, the analysis of next-generation sequencing data might be challenging for researchers who have never approached the study of viral ... -
A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit
(Society for Experimental Biology, 2008-07)In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of ... -
A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
(Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
A computational study of the interaction of the foot and mouth disease virus VP1 with monoclonal antibodies
(Elsevier, 2015-10)Foot and mouth disease is caused by a non-enveloped virus (FMDV), which disposes several antigenic sites at the surface of their capsid proteins. The most relevant and immunodominant antigenic site of FMDV (site A or AnSA) ... -
A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
(Nature Publishing Group, 2017-12)The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that ... -
A fijivirus major viroplasm protein shows RNA-stimulated ATPase activity by adopting pentameric and hexameric assemblies of dimers
(American Society for Microbiology, 2023-02)Fijiviruses replicate and package their genomes within viroplasms in a pro cess involving RNA-RNA and RNA-protein interactions. Here, we demonstrate that the 24 C-terminal residues (C-arm) of the P9-1 major viroplasm protein ... -
A fungal protease named AsES triggers antiviral immune responses and effectively restricts virus infection in arabidopsis and Nicotiana benthamiana plants
(Oxford University Press, 2022-04)Background and Aims: Plants have evolved complex mechanisms to fight against pathogens. Among these mechanisms, pattern-triggered immunity (PTI) relies on the recognition of conserved microbe- or pathogen-associated molecular ... -
A participatory surveillance of marsh deer (Blastocerus dichotomus) morbidity and mortality in Argentina: first results
(BMC, 2020-09)Background: In an era of unprecedented socio-ecological changes, managing wildlife health demands high-quality data collection and the engagement of local communities. Blastocerus dichotomus, the largest South American ... -
A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
(Hindawi, 2020-04)Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB ... -
A prime-boost combination of a three-protein cocktail and multiepitopic MVA as a vaccine against Babesia bigemina elicits neutralizing antibodies and a Th1 cellular immune response in mice
(Elsevier, 2022-09)In the intraerythrocytic protozoan parasites of the genus Babesia both innate and adaptive immune responses are necessary to confer protection against clinical disease. In particular, the adaptive immune response involves ... -
A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
(Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants
(Oxford University Press, 2018-11)Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol ... -
A transcriptional analysis of cattle immune cells reveals a central role of type 1 interferon in the In vitro innate immune response against Mycobacterium bovis
(MDPI, 2023-09)Bovine tuberculosis is a chronic infectious disease primarily caused by Mycobacterium bovis, a bacterium that affects cattle and other mammals, including humans. Despite the availability of vast research about the immune ... -
A β-Hairpin Motif in the Envelope Protein E2 Mediates Receptor Binding of Bovine Viral Diarrhea Virus
(MDPI, 2021-06)Pestivirus envelope protein E2 is crucial to virus infection and accomplishes virus-receptor interaction during entry. However, mapping of E2 residues mediating these interactions has remained unexplored. In this study, ... -
Achiote (Bixa orellana L.) : a natural source of pigment and vitamin E
(Springer, 2017-05)Commercialization of agricultural products, including seeds and its derived products, represents an important economic source for developing countries. Natural colorants obtained from the seeds of achiote plant (annatto) ... -
Acinetobacter baumannii and Its relationship to carbapenem resistance : a meta-analysis
(MDPI, 2022-05)Infections by antibiotic-resistant bacteria are a significant and complex global health issue. In this context, Acinetobacter baumannii is particularly important because of its ability to withstand treatments by β-lactams, ... -
AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
(Plos One, 2012-09)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ... -
Agroinoculation of a full-length cDNA clone of cotton leafroll dwarf virus (CLRDV) results in systemic infection in cotton and the model plant Nicotiana benthamiana
(2013-07)Cotton blue disease is the most important viral disease of cotton in the southern part of South America. Its etiological agent, cotton leafroll dwarf virus (CLRDV), is specifically transmitted to host plants by the aphid ... -
Aislamiento de Mycobacterium intracellulare a partir de un pulmón bovino comercializado en una carnicería de la zona oeste del conurbano bonaerense = Isolate of Mycobacterium intracellulare from a bovine lung marketed in a butcher shop from western of Greater Buenos Aires
(Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2016)Se realizó un muestreo de 62 pulmones bovinos en una carnicería y un frigorífico de la zona oeste del conurbano bonaerense. De uno de ellos, adquirido en una carnicería, y sin lesiones visibles compatibles con tuberculosis, ... -
Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
(2016)Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in ...