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Mostrando ítems 1-12 de 12
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A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
Trangoni, Marcos David; Gioffre, Andrea; Cravero, Silvio Lorenzo Pedro (Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
Brucella abortus: tipificación molecular por MLVA en aislamientos locales, análisis in silico de sus lipoproteínas e implicancia de las lipoproteínas en virulencia
Hollender, Daina (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2016-07)La brucelosis es una enfermedad zoonótica de distribución mundial, con un alto impacto en la salud pública y en la economía. De las 10 especies de Brucella reconocidas, B. abortus es la causante de la brucelosis bovina. ... -
Campylobacter fetus releases S-layered and immunoreactive outer membrane vesicles = Campylobacter fetus secreta vesículas de membrana externa inmunorreactivas que conservan la capa S
Farace, Pablo; Cravero, Silvio Lorenzo Pedro; Taibo, Catalina Beatriz; Diodati, Julian Angel; Morsella, Claudia Graciela; Paolicchi, Fernando; Sabio Y Garcia, Julia Veronica; Gioffre, Andrea (Elsevier, 2021-08)The study of outer membrane vesicles (OMVs) became relevant because of their probable important role in the transfer of virulence factors to host cells. Campylobacter fetus is mainly a mammal pathogen whose virulence ... -
Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
Ortiz Chura, Abimael; Marcoppido, Gisela Ariana; Gere, José; Depetris, Gustavo; Stefañuk Bahamonde, Francisco José; Trangoni, Marcos David; Cravero, Silvio Lorenzo Pedro; Faverin, Claudia; Cataldi, Angel Adrian; Ceron Cucchi, Maria Esperanza (Veterinary World Publisher, 2021-01)Background and Aim: Nitrate (NO3-) reduces enteric methane emissions and could be a source of non-protein nitrogen in ruminant feeds. Nonetheless, it has a potential toxic effect that could compromise animal health and ... -
Ciliate protozoa of the forestomach of llamas (Lama glama) from locations at different altitude in Argentina
Ceron Cucchi, Maria Esperanza; Marcoppido, Gisela Ariana; Morici, Gabriel Edgardo; Cravero, Silvio Lorenzo Pedro; Dekker, Anna; Fondevila, Manuel; De La Fuente, Gabriel (2016)This study describes the diversity and concentration of the protozoal population from the forestomach of llamas in Argentina at three altitudinal locations. Protozoal diversity was studied in samples from eight llamas from ... -
Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
Avila, Héctor Gabriel; Mozzoni, Cecilia; Trangoni, Marcos David; Cravero, Silvio Lorenzo Pedro; Pérez, Verónica Mirtha; Valenzuela, Federico; Gertiser, María Laura; Butti, Marcos Javier; Kamenetzky, Laura; Jensen, Oscar; Rosenzvit, Mara Cecilia (Elsevier, 2020-01)Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is ... -
Dynamics of the ruminal microbial ecosystem, and inhibition of methanogenesis and propiogenesis in response to nitrate feeding to Holstein calves
Ortiz Chura, Abimael; Gere, José; Marcoppido, Gisela Ariana; Depetris, Gustavo; Cravero, Silvio Lorenzo Pedro; Faverin, Claudia; Pinares-Patiño, Cesar; Cataldi, Angel Adrian; Ceron Cucchi, Maria Esperanza (Elsevier, 2021-12)It is known that nitrate inhibits ruminal methanogenesis, mainly through competition with hydrogenotrophic methanogens for available hydrogen (H2) and also through toxic effects on the methanogens. However, there is limited ... -
La emisión de metano entérico en sistemas de producción ganadera
Ceron Cucchi, Maria Esperanza; Gere, Jose Ignacio; Ortiz Chura, Abimael; Cravero, Silvio Lorenzo Pedro; Nieto, Maria Isabel (Asociación Peruana de Producción Animal, 2018-12)La ganadería mundial es una de las principales fuentes de las emisiones antropogénicas de gases de efecto invernadero (GEI), ya que representa cerca del 18% a nivel global y con estimaciones de seguir creciendo en las ... -
Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
Grilli, Diego Javier; Ceron Cucchi, Maria Esperanza; Paez Lama, Sebastián Antonio; Egea, Angela Vanina; Schnittger, Leonhard; Cravero, Silvio Lorenzo Pedro; Sosa Escudero, Miguel Angel; Allegretti, Liliana Inés; Arenas, Graciela Nora (Springer, 2013-09)We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
Farace, Pablo Daniel; Morsella, Claudia Graciela; Cravero, Silvio Lorenzo Pedro; Sioya, Bernardo Arturo; Amadio, Ariel; Paolicchi, Fernando; Gioffre, Andrea (2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Trangoni, Marcos David; Gioffre, Andrea; Ceron Cucchi, Maria Esperanza; Caimi, Karina Cynthia; Ruybal, Paula; Zumarraga, Martin Jose; Cravero, Silvio Lorenzo Pedro (Sociedade Brasileira de Microbiologia, 2015-06)In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific ... -
Llama and sheep ruminal fluid digestive capacity by in vitro gas production technique
Ortiz Chura, Abimael; Wawrzkiewicz, Marisa; Cravero, Silvio Lorenzo Pedro; Fernández Pepi, María Gabriela; Ceron Cucchi, Maria Esperanza; Jaurena, Gustavo (Wiley, 2022-03)BACKGROUND: The in vitro gas production technique has been used to evaluate forage fermentation kinetics. However, individual and animal species variation can change fermentation patterns due to differences in ruminal ...