resumen
Abstract
In this study, we identified candidate genes associated with the reproductive success of Varroa destructor in Apis mellifera brood cells at a key point in time, the 4th day after host cell capping. First-life cycle reproducing (R) and non-reproducing (NR) mites obtained by applying a semi-field rearing protocol were analyzed by RNA-Seq and qPCR. We observed a general under-expression of genes associated with metabolism and a few over-expressed genes
[ver mas...]
dc.contributor.author | Muntaabski, Irina | |
dc.contributor.author | Latorre Estivalis, Jose M. | |
dc.contributor.author | Russo, Romina Maria | |
dc.contributor.author | Landi, Lucas | |
dc.contributor.author | Agra, Marcelo Nicolás | |
dc.contributor.author | Liendo, María Clara | |
dc.contributor.author | Lanzavecchia, Silvia Beatriz | |
dc.contributor.author | Scannapieco, Alejandra Carla | |
dc.date.accessioned | 2023-10-18T13:38:48Z | |
dc.date.available | 2023-10-18T13:38:48Z | |
dc.date.issued | 2023-10 | |
dc.identifier.issn | 1297-9678 | |
dc.identifier.other | https://doi.org/10.1007/s13592-023-01027-7 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/15577 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s13592-023-01027-7 | |
dc.description.abstract | In this study, we identified candidate genes associated with the reproductive success of Varroa destructor in Apis mellifera brood cells at a key point in time, the 4th day after host cell capping. First-life cycle reproducing (R) and non-reproducing (NR) mites obtained by applying a semi-field rearing protocol were analyzed by RNA-Seq and qPCR. We observed a general under-expression of genes associated with metabolism and a few over-expressed genes putatively involved in signal transduction and DNA-binding transcription factor activity in R compared to NR mites. Specifically, PTCH1 and AP-1 genes, associated with embryonic segmentation and apoptosis, emerged as the best candidates for reproductive success in R mites and the early abortion of reproduction in NR mites. We also detected differentially transcribed long non-coding RNAs between R and NR mites with cis target genes annotated as regulators of replication, transcription, and translation pathways. Some of these genes were specifically associated with embryonic development and apoptosis. We discuss putative metabolic pathways associated with V. destructor reproduction to provide novel information for developing innovative and environmentally friendly mite control strategies. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagas | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Apidologie 54 (5) : 49 (Octubre 2023) | es_AR |
dc.subject | Varroa destructor | es_AR |
dc.subject | Reprouction Control | eng |
dc.subject | Reproducción Dirigida | es_AR |
dc.subject | Embryonic Development | eng |
dc.subject | Desarrollo Embrionario | es_AR |
dc.subject | Host Parasite Relations | eng |
dc.subject | Relaciones Huésped Parásito | es_AR |
dc.subject | Gene Expression | eng |
dc.subject | Expresión Génica | es_AR |
dc.subject | RNA Sequence | eng |
dc.subject | Secuencia de ARN | es_AR |
dc.subject | Apoptosis | eng |
dc.subject | Candidate Genes | eng |
dc.subject | Genes Candidatos | es_AR |
dc.subject.other | Embryogenesis | eng |
dc.subject.other | Embriogénesis | es_AR |
dc.title | Embryogenesis and apoptosis appears as key molecular pathways involved in Varroa destructor reproduction | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Genética | es_AR |
dc.description.fil | Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Latorre Estivalis, Jose M. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina | es_AR |
dc.description.fil | Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Agra, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad Integrada INTA-Universidad Nacional de Mar del Plata; Argentina | es_AR |
dc.description.fil | Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
Files in this item
This item appears in the following Collection(s)
common
-
Artículos científicos [168]