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Aislamiento de Mycobacterium intracellulare a partir de un pulmón bovino comercializado en una carnicería de la zona oeste del conurbano bonaerense = Isolate of Mycobacterium intracellulare from a bovine lung marketed in a butcher shop from western of Greater Buenos Aires
(Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2016)Se realizó un muestreo de 62 pulmones bovinos en una carnicería y un frigorífico de la zona oeste del conurbano bonaerense. De uno de ellos, adquirido en una carnicería, y sin lesiones visibles compatibles con tuberculosis, ... -
Development of a droplet digital PCR assay for quantification of the proviral load of bovine leukemia virus
(Sage Publications, 2022-05)Droplet digital PCR (ddPCR) is a highly sensitive tool developed for the detection and quantification of short-sequence variants—a tool that offers unparalleled precision enabling measurement of smaller-fold changes. We ... -
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
(2017-12)Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have ... -
Diferenciación de serovares de leptospiras patógenas mediante PCR del gen ligB y secuenciación = Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing
(Asociación Argentina de Microbiología, 2018)La leptospirosis es la zoonosis de mayor distribución mundial. El objetivo del presente trabajo fue desarrollar una técnica molecular que diferencie leptospiras patógenas. Se amplificó mediante PCR y se secuenció una ... -
Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices : implications for defining molecular markers of cell integrity
(2017-07)In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or ... -
International interlaboratory validation of a nested PCR for molecular detection of Babesia bovis and Babesia bigemina, causative agents of bovine babesiosis
(Elsevier, 2022-04)Babesia bovis and B. bigemina are tick-transmitted parasites causing bovine babesiosis, characterized by significant morbidity and mortality leading to economic losses to the livestock industry in tropical and subtropical ... -
Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates
(BMC, 2013-06)Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to understand the ... -
Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters
(Springer, 2024-03)Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated ...