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Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of
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dc.contributor.author | Hidalgo-Ruiz, Mario | |
dc.contributor.author | Mejia-López, Susana | |
dc.contributor.author | Pérez-Serrano, Rosa M. | |
dc.contributor.author | Zaldívar-Lelo de Larrea, Guadalupe | |
dc.contributor.author | Ganzinelli, Sabrina Belen | |
dc.contributor.author | Florin-Christensen, Monica | |
dc.contributor.author | Suarez, Carlos Esteban | |
dc.contributor.author | Hernández-Ortiz, Rubén | |
dc.contributor.author | Mercado-Uriostegui, Miguel A. | |
dc.contributor.author | Rodríguez-Torres, Angelina | |
dc.contributor.author | Carvajal-Gamez, Bertha I. | |
dc.contributor.author | Camacho-Nuez, Minerva | |
dc.contributor.author | Wilkowsky, Silvina Elizabeth | |
dc.contributor.author | Mosqueda, Juan | |
dc.date.accessioned | 2022-04-07T10:43:44Z | |
dc.date.available | 2022-04-07T10:43:44Z | |
dc.date.issued | 2022-02 | |
dc.identifier.issn | 0264-410X | |
dc.identifier.other | https://doi.org/10.1016/j.vaccine.2022.01.023 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/11574 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0264410X22000494 | |
dc.description.abstract | Vaccines against bovine babesiosis must, ideally, induce a humoral immune response characterized by neutralizing antibodies against conserved epitopes and a cellular Th1 immune response. In Babesia bovis, proteins such as AMA-1, MSA-2c, and RAP-1 have been characterized and antibodies against these proteins have shown a neutralizing effect, demonstrating the implication of B and T-cell epitopes in the immune response. There is evidence of the existence of B and T-cell epitopes in these proteins, however, it remains to be defined, the presence of conserved peptides in strains from around the world containing B and T-cell epitopes, and their role in the generation of a long-lasting immunity. The aim in this paper was to identify peptides of Babesia bovis AMA-1, MSA-2c, and RAP-1 that elicit a neutralizing and long-lasting Th1 immune response. Peptides containing B-cell epitopes of AMA-1, MSA-2c and RAP-1, were identified. The immune response generated by each peptide was characterized in cattle. All peptides tested induced antibodies that recognized intraerythrocytic parasites, however, only 5 peptides generated neutralizing antibodies in vitro: P2AMA-1 (6.28%), P3MSA-2c (10.27%), P4MSA-2c (10.42%), P1RAP-1 (32.45%), and P4RAP-1 (36.98%). When these neutralizing antibodies were evaluated as a pool, the inhibition percentage of invasion increased to 52.37%. When the T cellular response was evaluated, two peptides: P3MSA2c and P2AMA1 induced a higher percentage (>70%) of activated CD4 +/CD45RO+ T cells than unstimulated cells. Additionally, both peptides induced the production of gamma interferon (IFN-) in PBMCs from vaccinated cattle after one year proving the implication of a long-lasting Th1 immune response. In conclusion, we identified conserved peptides containing B and T-cell epitopes in antigens of B. bovis that elicit a Th1 immune response and showed evidence that peptides from the same protein elicit different immune responses, which has implication for vaccine development in bovine babesiosis. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Vaccine 40 (8) : 1108-1115 (Febrero 2022) | es_AR |
dc.subject | Babesia bovis | es_AR |
dc.subject | T-lymphocytes | eng |
dc.subject | Linfocitos-t | es_AR |
dc.subject | Interferons | eng |
dc.subject | Interferonas | es_AR |
dc.subject | Antibodies | eng |
dc.subject | Anticuerpos | es_AR |
dc.subject | Immune Response | eng |
dc.subject | Respuesta Inmunológica | es_AR |
dc.subject | Neutralization | eng |
dc.subject | Neutralización | es_AR |
dc.subject.other | T-cell | eng |
dc.subject.other | Células-t | es_AR |
dc.title | Babesia bovis AMA-1, MSA-2c and RAP-1 contain conserved B and T-cell epitopes, which generate neutralizing antibodies and a long-lasting Th1 immune response in vaccinated cattle | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Patobiología | es_AR |
dc.description.fil | Fil: Hidalgo-Ruiz, Mario. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México | es_AR |
dc.description.fil | Fil: Mejia-López, Susana. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México | es_AR |
dc.description.fil | Fil: Pérez-Serrano, Rosa M. Universidad Autónoma de Querétaro. Facultad de Medicina; México | es_AR |
dc.description.fil | Fil: Zaldívar-Lelo de Larrea, Guadalupe. Universidad Autónoma de Querétaro. Facultad de Medicina; México | es_AR |
dc.description.fil | Fil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina | es_AR |
dc.description.fil | Fil: Ganzinelli Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria (IPVET); Argentina | es_AR |
dc.description.fil | Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Suarez, Carlos Esteban. United States Department of Agricultural-Agricultural Research Service. Animal Disease Research Unit; Estados Unidos | es_AR |
dc.description.fil | Fil: Hernández-Ortiz, Rubén. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Centro de Estudios e Investigaciones para el Desarrollo Docente (CENID). Salud Animal e Inocuidad; México | es_AR |
dc.description.fil | Fil: Mercado-Uriostegui, Miguel A. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México | es_AR |
dc.description.fil | Fil: Rodríguez-Torres, Angelina. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México | es_AR |
dc.description.fil | Fil: Carvajal-Gamez, Bertha I. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México | es_AR |
dc.description.fil | Fil: Camacho-Nuez, Minerva. Universidad Autónoma de la Ciudad de México. Posgrado en Ciencias Genómicas; México | es_AR |
dc.description.fil | Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Mosqueda, Juan. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México | es_AR |
dc.subtype | cientifico |
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