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Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin [ver mas...]
dc.contributor.authorConforto, Erica Cinthia
dc.contributor.authorBernanrdi Lima, Nelson
dc.contributor.authorGarcete-Gómez, J. M.
dc.contributor.authorCâmara, M. P. S.
dc.contributor.authorMichereff, S. J.
dc.date.accessioned2021-07-13T10:29:36Z
dc.date.available2021-07-13T10:29:36Z
dc.date.issued2016-03-18
dc.identifier.issn0191-2917
dc.identifier.issn1943-7692 (online)
dc.identifier.otherhttps://doi.org/10.1094/PDIS-11-15-1298-PDN
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9790
dc.identifier.urihttps://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN
dc.description.abstractCactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608).eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherAmerican Phytopathological Societyes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourcePlant Disease 100 (6) : 1238 (June 2016)es_AR
dc.subjectOpuntiaes_AR
dc.subjectCladodeseng
dc.subjectBrasiles_AR
dc.subjectOpuntia Ficus Indicaes_AR
dc.subjectCladodios
dc.subject.otherPrickly Peareng
dc.titleFirst Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Braziles_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentinaes_AR
dc.description.filFil: Bernardi Lima, Nelson. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasiles_AR
dc.description.filFil: Garcete-Gómez, José María. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasiles_AR
dc.description.filFil: Câmara, M. P. S. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasiles_AR
dc.description.filFil: Michereff, S. J. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasiles_AR
dc.subtypecientifico


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