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Abstract
Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source [ver mas...]
dc.contributor.authorZanini, Andrea Alejandra
dc.contributor.authorDi Feo, Liliana Del Valle
dc.contributor.authorLuna, Dario Fernando
dc.contributor.authorPaccioretti, Pablo
dc.contributor.authorCollavino, Agostina
dc.contributor.authorRodriguez, Marianela
dc.date.accessioned2021-06-25T16:16:52Z
dc.date.available2021-06-25T16:16:52Z
dc.date.issued2021-01
dc.identifier.issn0032-0862
dc.identifier.issn1365-3059 (online)
dc.identifier.otherhttps://doi.org/10.1111/ppa.13272
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9664
dc.identifier.urihttps://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13272
dc.description.abstractCassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourcePlant Pathology 70 (1) : 195-205 (January 2021)es_AR
dc.subjectBiotic Stresseng
dc.subjectCassavaeng
dc.subjectPotexviruseseng
dc.subjectEstrés Bióticoes_AR
dc.subjectMandiocaes_AR
dc.subjectCloroplastoes_AR
dc.subjectPotexviruses_AR
dc.subject.otherChloroplast Alterationeng
dc.subject.otherCsCMVeng
dc.subject.otherOJIP Testeng
dc.subject.otherPotexviruses_AR
dc.titleCassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plantses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Fisiología y Recursos Genéticos Vegetaleses_AR
dc.description.filFil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentinaes_AR
dc.description.filFil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentinaes_AR
dc.description.filFil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Di Feo, Liliana Del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentinaes_AR
dc.description.filFil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentinaes_AR
dc.description.filFil: Luna, Dario Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentinaes_AR
dc.description.filFil: Paccioretti, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentinaes_AR
dc.description.filFil: Collavino, Agostina. Instituto Universitario de Formosa. Facultad de la Producción y del Medio Ambiente. Centro de Investigación y Transferencia de Formosa; Argentinaes_AR
dc.description.filFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentinaes_AR
dc.description.filFil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentinaes_AR
dc.subtypecientifico


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