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resumen

Resumen
Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages [ver mas...]
dc.contributor.authorLeiva, Carlos Leónidas
dc.contributor.authorGeoghegan, Patricia
dc.contributor.authorLammer, Mónica
dc.contributor.authorCangelosi, Adriana
dc.contributor.authorMariconda, Virginia
dc.contributor.authorCeli Castillo, Ana Beatriz
dc.contributor.authorBrero, Luisa
dc.contributor.authorChacana, Pablo
dc.date.accessioned2021-05-05T16:38:06Z
dc.date.available2021-05-05T16:38:06Z
dc.date.issued2021-07
dc.identifier.issn1872-9142
dc.identifier.otherhttps://doi.org/10.1016/j.molimm.2021.04.015
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9280
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0161589021001322
dc.description.abstractBee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceMolecular Immunology 135 : 183-190 (Julio 2021)es_AR
dc.subjectApis melliferaes_AR
dc.subjectBee Venomseng
dc.subjectVeneno de Abejaes_AR
dc.subjectAntibodieseng
dc.subjectAnticuerposes_AR
dc.subjectEgg Yolkeng
dc.subjectYema de Huevoes_AR
dc.subjectIn vivo Experimentationeng
dc.subjectExperimentación in Vivoes_AR
dc.subjectLethal Effectseng
dc.subjectEfectos Letaleses_AR
dc.subjectNeutralizationeng
dc.subjectNeutralizaciónes_AR
dc.subject.otherIgY Antibodieseng
dc.subject.otherAnticuerpos IgYes_AR
dc.titleIn vivo neutralization of bee venom lethality by IgY antibodieses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentinaes_AR
dc.description.filFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentinaes_AR
dc.description.filFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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