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Resumen
Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages
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dc.contributor.author | Leiva, Carlos Leónidas | |
dc.contributor.author | Geoghegan, Patricia | |
dc.contributor.author | Lammer, Mónica | |
dc.contributor.author | Cangelosi, Adriana | |
dc.contributor.author | Mariconda, Virginia | |
dc.contributor.author | Celi Castillo, Ana Beatriz | |
dc.contributor.author | Brero, Luisa | |
dc.contributor.author | Chacana, Pablo | |
dc.date.accessioned | 2021-05-05T16:38:06Z | |
dc.date.available | 2021-05-05T16:38:06Z | |
dc.date.issued | 2021-07 | |
dc.identifier.issn | 1872-9142 | |
dc.identifier.other | https://doi.org/10.1016/j.molimm.2021.04.015 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/9280 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0161589021001322 | |
dc.description.abstract | Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Molecular Immunology 135 : 183-190 (Julio 2021) | es_AR |
dc.subject | Apis mellifera | es_AR |
dc.subject | Bee Venoms | eng |
dc.subject | Veneno de Abeja | es_AR |
dc.subject | Antibodies | eng |
dc.subject | Anticuerpos | es_AR |
dc.subject | Egg Yolk | eng |
dc.subject | Yema de Huevo | es_AR |
dc.subject | In vivo Experimentation | eng |
dc.subject | Experimentación in Vivo | es_AR |
dc.subject | Lethal Effects | eng |
dc.subject | Efectos Letales | es_AR |
dc.subject | Neutralization | eng |
dc.subject | Neutralización | es_AR |
dc.subject.other | IgY Antibodies | eng |
dc.subject.other | Anticuerpos IgY | es_AR |
dc.title | In vivo neutralization of bee venom lethality by IgY antibodies | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Patobiología | es_AR |
dc.description.fil | Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina | es_AR |
dc.description.fil | Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina | es_AR |
dc.description.fil | Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina | es_AR |
dc.description.fil | Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina | es_AR |
dc.description.fil | Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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