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resumen

Resumen
Despite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatility, we tested the hypothesis that species or genotypes of Bradyrhizobium could be a proxy for GP degrader potential in soil. A quantitative PCR assay was [ver mas...]
dc.contributor.authorHernandez Guijarro, Keren
dc.contributor.authorDe Geronimo, Eduardo
dc.contributor.authorErijman, Leonardo
dc.date.accessioned2021-04-21T11:32:40Z
dc.date.available2021-04-21T11:32:40Z
dc.date.issued2021-04-02
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.otherhttps://doi.org/10.1007/s00284-021-02467-z
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9141
dc.identifier.urihttps://link.springer.com/article/10.1007/s00284-021-02467-z
dc.description.abstractDespite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatility, we tested the hypothesis that species or genotypes of Bradyrhizobium could be a proxy for GP degrader potential in soil. A quantitative PCR assay was designed to target a specific region of the glycine oxidase gene (thiO), involved in the oxidation of glyphosate to AMPA, from known sequences of Bradyrhizobium species. The abundance of the thiO gene was determined in response to herbicide application in soils with different GP exposure history both under field and microcosm conditions. The gene coding for RNA polymerase subunitB (rpoB) was used as a reference for the abundance of total Bradyrhizobia. The assay using the designed primers was linear over a very large concentration range of the target and showed high efficiency and specificity. In a field experiment, there was a differential response related to the history of glyphosate use and the native Bradyrhizobium genotypes. In a soil without previous exposure to herbicides, thiO gene increased over time after glyphosate application with most genotypes belonging to the B. jicamae and B. elkanni supergroups. Conversely, in an agricultural soil with more than 10 years of continuous glyphosate application, the abundance of thiO gene decreased and most genotypes belonged to B. japonicum supergroup. In a microcosm assay, the amount of herbicide degraded after a single application was positively correlated to the number of thiO copies in different agricultural soils from the Pampean Region. Our results suggest that Bradyrhizobium species are differently involved in glyphosate degradation, denoting the existence of metabolically versatile microorganisms which can be explored for sustainable agriculture practices. The relationship between the abundance of thiO gene and the GP degraded in soil point to the use of thiO gene as a proxy for GP degradation in soil.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringer Naturees_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E2-I039-002/2019-PD-E2-I039-002/AR./REMEDIACIÓN DE SUELOS Y AGUAS Y RESTAURACIÓN ECOLÓGICA DE SISTEMAS DEGRADADOS POR USO AGROPECUARIO, AGROINDUSTRIAL Y ACTIVIDADES EXTRACTIVAS
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceCurrent Microbiology (2021)es_AR
dc.subjectMicroorganismoses_AR
dc.subjectMicroorganismseng
dc.subjectBradyrhizobiumes_AR
dc.subjectDegradación del Sueloes_AR
dc.subjectSoil Degradationeng
dc.subjectGlifosatoes_AR
dc.subjectGlyphosateeng
dc.titleGlyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobiumes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: De Gerónimo, Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: De Gerónimo, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Erijman, Leonardo. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.es_AR
dc.subtypecientifico


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