Mostrar el registro sencillo del ítem

resumen

Resumen
Plant responses to NH4 + stress are complex, and multiple mechanisms underlying NH4 + sensitivity and tolerance in plants may be involved. Here, we demonstrate that macro‐ and microautophagic activities are oppositely affected in plants grown under NH4 + toxicity conditions. When grown under NH4 + stress conditions, macroautophagic activity was impaired in roots. Root cells accumulated autophagosomes in the cytoplasm, but showed less autophagic flux, [ver mas...]
dc.contributor.authorRobert, German
dc.contributor.authorYagyu, Mako
dc.contributor.authorKoizumi, Takaya
dc.contributor.authorNaya, Loreto
dc.contributor.authorMasclaux‐Daubresse, Céline
dc.contributor.authorYoshimoto, Kohki
dc.date.accessioned2021-02-26T11:39:46Z
dc.date.available2021-02-26T11:39:46Z
dc.date.issued2020-11-22
dc.identifier.issn0960-7412
dc.identifier.issn1365-313X (online)
dc.identifier.otherhttps://doi.org/10.1111/tpj.15091
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8757
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1111/tpj.15091
dc.description.abstractPlant responses to NH4 + stress are complex, and multiple mechanisms underlying NH4 + sensitivity and tolerance in plants may be involved. Here, we demonstrate that macro‐ and microautophagic activities are oppositely affected in plants grown under NH4 + toxicity conditions. When grown under NH4 + stress conditions, macroautophagic activity was impaired in roots. Root cells accumulated autophagosomes in the cytoplasm, but showed less autophagic flux, indicating that late steps of the macroautophagy process are affected under NH4 + stress conditions. Under this scenario, we also found that the CCZ1‐MON1 complex, a critical factor for vacuole delivery pathways, functions in the late step of the macroautophagic pathway in Arabidopsis. In contrast, an accumulation of tonoplast‐derived vesicles was observed in vacuolar lumens of root cells of NH4 +‐stressed plants, suggesting the induction of a microautophagy‐like process. In this sense, some SYP22‐, but mainly VAMP711‐positive vesicles were observed inside vacuole in roots of NH4 +‐stressed plants. Consistent with the increased tonoplast degradation and the reduced membrane flow to the vacuole due to the impaired macroautophagic flux, the vacuoles of root cells of NH4 +‐stressed plants showed a simplified structure and lower tonoplast content. Taken together, this study presents evidence that postulates late steps of the macroautophagic process as a relevant physiological mechanism underlying the NH4 + sensitivity response in Arabidopsis, and additionally provides insights into the molecular tools for studying microautophagy in plants.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceThe Plant Journal 105 (4) : 1083-1097 (February 2021)es_AR
dc.subjectAmmonium Nitrogeneng
dc.subjectToxicityeng
dc.subjectVacuoleseng
dc.subjectArabidopsises_AR
dc.subjectStresseng
dc.subjectNitrógeno Amoniacal
dc.subjectToxicidad
dc.subjectVacúola
dc.subjectEstres
dc.subject.otherNH4-Nes_AR
dc.subject.otherNH4es_AR
dc.subject.otherMacroautophagyeng
dc.subject.otherMicroautophagyeng
dc.subject.otherAutophagosome-to-vacuole Fusioneng
dc.subject.otherCCZ1-MON1es_AR
dc.subject.otherVacuole Morphologyeng
dc.titleAmmonium stress increases microautophagic activity while impairing macroautophagic flux in Arabidopsis rootses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Fisiología y Recursos Genéticos Vegetaleses_AR
dc.description.filFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios (UDEA); Argentina. Université Paris‐Saclay. Institut Jean‐Pierre Bourgin, INRAE, AgroParisTech; Francees_AR
dc.description.filFil: Yagyu, Mako. Meiji University. School of Agriculture. Department of Life Sciences; Japónes_AR
dc.description.filFil: Koizumi, Takaya. Meiji University. School of Agriculture. Department of Life Sciences; Japónes_AR
dc.description.filFil: Naya, Loreto. Université Paris‐Saclay. Institut Jean‐Pierre Bourgin, INRAE, AgroParisTech; Franciaes_AR
dc.description.filFil: Masclaux‐Daubresse, Céline. Université Paris‐Saclay. Institut Jean‐Pierre Bourgin, INRAE, AgroParisTech; Franciaes_AR
dc.description.filFil: Yoshimoto, Kohki. Université Paris‐Saclay. Institut Jean‐Pierre Bourgin, INRAE, AgroParisTech; Francia. Meiji University. School of Agriculture. Department of Life Sciences; Japónes_AR
dc.subtypecientifico


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

common

Mostrar el registro sencillo del ítem