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Abstract
Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. [ver mas...]
dc.contributor.authorIribarren, Maria Josefina
dc.contributor.authorPascuan, Cecilia Gabriela
dc.contributor.authorSoto, Gabriela Cynthia
dc.contributor.authorAyub, Nicolás Daniel
dc.date.accessioned2021-02-23T13:25:34Z
dc.date.available2021-02-23T13:25:34Z
dc.date.issued2015-11
dc.identifier.issn1574-6968
dc.identifier.otherhttps://doi.org/10.1093/femsle/fnv189
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8727
dc.identifier.urihttps://academic.oup.com/femsle/article/362/22/fnv189/1816015
dc.description.abstractPhytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. The occurrence of PcAVR3a1 in environmental strains of P. capsici was confirmed by PCR in only 21 of these pathogen isolates. To understand the presence-absence pattern of PcAVR3a1 in environmental strains, the flanking region of this gene was sequenced. PcAVR3a1 was found within a genetic element that we named PcAVR3a1-GI (PcAVR3a1 genomic island). PcAVR3a1-GI was flanked by a 22-bp direct repeat, which is related to its site-specific recombination site. In addition to the PcAVR3a1 gene, PcAVR3a1-GI also encoded a phage integrase probably associated with the excision and integration of this mobile element. Exposure to plant induced the presence of an episomal circular intermediate of PcAVR3a1-GI, indicating that this mobile element is functional. Collectively, these findings provide evidence of PcAVR3a1 evolution via mobile elements in environmental strains of Phytophthora.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherOxford University Presses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceFEMS Microbiology Letters 362 (22) : fnv189 (Octubre 2015)es_AR
dc.subjectPhytophthora capsicies_AR
dc.subjectVegetable Cropseng
dc.subjectHortalizas (plantas)es_AR
dc.subjectPathogenseng
dc.subjectOrganismos Patógenoses_AR
dc.subjectEffectorseng
dc.subjectEfectores Moleculareses_AR
dc.subjectGenomicseng
dc.subjectGenómicaes_AR
dc.titleGenetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic islandes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Iribarren, Maria Josefina. Universidad Nacional de Luján; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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