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Abstract
Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large [ver mas...]
dc.contributor.authorCarabajal Paladino, Leonale Suzel
dc.contributor.authorPapeschi, Alba Graciela
dc.contributor.authorLanzavecchia, Silvia Beatriz
dc.contributor.authorCladera, Jorge Luis
dc.contributor.authorBressa, María José
dc.date.accessioned2021-02-23T12:59:01Z
dc.date.available2021-02-23T12:59:01Z
dc.date.issued2013-07
dc.identifier.issn1802-8829
dc.identifier.otherhttps://doi.org/10.14411/eje.2013.054
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8725
dc.identifier.urihttps://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php
dc.description.abstractDiachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherCzech Academy of Scienceses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceEuropean Journal of Entomology 110 (3) : 401-409 (Julio 2013)es_AR
dc.subjectHymenopteraes_AR
dc.subjectBraconidaees_AR
dc.subjectBiosteres longicaudatuses_AR
dc.subjectKaryotypeseng
dc.subjectCariotiposes_AR
dc.subjectHeterochromatineng
dc.subjectHeterocromatinaes_AR
dc.subjectRibosomal DNAeng
dc.subjectADN ribosomales_AR
dc.subjectCytogeneticseng
dc.subjectCitogenéticaes_AR
dc.subjectParasitoidseng
dc.subjectParasitoideses_AR
dc.subjectBiological Control Agentseng
dc.subjectAgentes de Control Biológicoes_AR
dc.subject.otherDiachasmimorpha longicaudataes_AR
dc.titleCytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agentes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Carabajal Paladino, Leonela Zusel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentinaes_AR
dc.description.filFil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentinaes_AR
dc.description.filFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentinaes_AR
dc.description.filFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentinaes_AR
dc.description.filFil: Bressa, Maria Jose. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentinaes_AR
dc.subtypecientifico


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