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Abstract
Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants [ver mas...]
dc.contributor.authorAyub, Nicolás Daniel
dc.contributor.authorFox, Ana Romina
dc.contributor.authorGarcia, Araceli Nora
dc.contributor.authorMozzicafreddo, Matteo
dc.contributor.authorCuccioloni, Massimiliano
dc.contributor.authorAngeletti, Mauro
dc.contributor.authorPagano, Elba Maria
dc.contributor.authorSoto, Gabriela Cynthia
dc.date.accessioned2021-02-22T12:43:22Z
dc.date.available2021-02-22T12:43:22Z
dc.date.issued2015-01
dc.identifier.issn1574-6968
dc.identifier.otherhttps://doi.org/10.1093/femsle/fnu006
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8712
dc.identifier.urihttps://academic.oup.com/femsle/article/362/2/1/2467374
dc.description.abstractSnakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherOxford University Presses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceFEMS Microbiology Letters 362 (2) : 1-6 (Enero 2015)es_AR
dc.subjectImmunityeng
dc.subjectInmunidades_AR
dc.subjectAntimicrobial Agentseng
dc.subjectAgente Antimicrobianoes_AR
dc.subjectPeptideseng
dc.subjectPéptidoses_AR
dc.subjectPseudomonases_AR
dc.subjectDefence Mechanismseng
dc.subjectMecanismos de Defensaes_AR
dc.subjectMedicago sativaes_AR
dc.subject.otherAlfalfa
dc.titlePseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Genéticaes_AR
dc.description.filFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Mozzicafreddo, Matteo. University of Camerino. School of Biosciences and Biotechnology; Italiaes_AR
dc.description.filFil: Cuccioloni, Massimiliano. University of Camerino. School of Biosciences and Biotechnology; Italiaes_AR
dc.description.filFil: Angeletti, Mauro. University of Camerino. School of Biosciences and Biotechnology; Italiaes_AR
dc.description.filFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentinaes_AR
dc.description.filFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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