Show simple item record

resumen

Abstract
Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M [ver mas...]
dc.contributor.authorMartinez, Mara Leila
dc.contributor.authorRodriguez, Marcelo A.
dc.contributor.authorIrazu, Lucía E.
dc.contributor.authorRomero, Graciela Noemi
dc.contributor.authorSaraullo, Vanina Rosa
dc.contributor.authorWatanabe, Olivia
dc.contributor.authorHamer, Micaela
dc.contributor.authorGrune Loffler, Sylvia
dc.contributor.authorSamartino, Luis Ernesto
dc.contributor.authorBrihuega, Bibiana Felicitas
dc.date.accessioned2021-01-26T15:01:16Z
dc.date.available2021-01-26T15:01:16Z
dc.date.issued2021-04
dc.identifier.issn1878-1667
dc.identifier.otherhttps://doi.org/10.1016/j.cimid.2021.101609
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8652
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0147957121000011
dc.description.abstractLeptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine.eng
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceComparative immunology, microbiology and infectious ddiseases 75 : 101609. (Abril 2021)es_AR
dc.subjectELISAes_AR
dc.subjectBovinaees_AR
dc.subjectLeptospiraes_AR
dc.subjectLeptospirosises_AR
dc.subjectDiagnosiseng
dc.subjectDiagnósticoes_AR
dc.subjectAbortioneng
dc.subjectAbortoes_AR
dc.subjectEnzymeseng
dc.subjectEnzimases_AR
dc.subjectImmunological Techniqueseng
dc.subjectTécnicas Inmunológicases_AR
dc.subjectAntibodieseng
dc.subjectAnticuerposes_AR
dc.titleNew enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine seraeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Patobiologíaes_AR
dc.description.filFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentinaes_AR
dc.description.filFil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentinaes_AR
dc.description.filFil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.es_AR
dc.description.filFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record