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Abstract
The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 [ver mas...]
dc.contributor.authorFerron, François
dc.contributor.authorDebat, Humberto Julio
dc.contributor.authorShannon, Ashleigh
dc.contributor.authorDecroly, Etienne
dc.contributor.authorCanard, Bruno
dc.date.accessioned2020-12-02T17:17:47Z
dc.date.available2020-12-02T17:17:47Z
dc.date.issued2019-12-19
dc.identifier.issn2631-9268 (online)
dc.identifier.otherhttps://doi.org/10.1093/nargab/lqz022
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8369
dc.identifier.urihttps://academic.oup.com/nargab/article/2/1/lqz022/5680725
dc.description.abstractThe order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherOxford University Press (OUP)es_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceNAR Genomics and Bioinformatics 2 (1) : lqz022 (March 2020)es_AR
dc.subjectCoronaviridaees_AR
dc.subjectViruseseng
dc.subjectNidoviraleses_AR
dc.subjectGenetic Markerseng
dc.subjectRNAeng
dc.subjectARN
dc.subjectMarcadores Genéticos
dc.subjectVirus
dc.titleA N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridaees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Ferron, François. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemaniaes_AR
dc.description.filFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentinaes_AR
dc.description.filFil: Shannon, Ashleigh. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Franciaes_AR
dc.description.filFil: Decroly, Etienne. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Franciaes_AR
dc.description.filFil: Canard, Bruno. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemaniaes_AR
dc.subtypecientifico


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