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Abstract
In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured [ver mas...]
dc.contributor.authorGoytia Bertero, Valentina
dc.contributor.authorBeznec, Ailin
dc.contributor.authorFaccio, Paula Daniela
dc.contributor.authorAuteri, Micol Tais
dc.contributor.authorArteaga, Martin
dc.contributor.authorBonafede, Marcos
dc.contributor.authorBossio, Maria Emilia
dc.date.accessioned2020-10-28T22:16:35Z
dc.date.available2020-10-28T22:16:35Z
dc.date.issued2020-07-30
dc.identifier.issn1054-5476
dc.identifier.otherhttps://doi.org/10.1007/s11627-020-10098-5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8145
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs11627-020-10098-5
dc.description.abstractIn vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis).eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceIn Vitro Cellular & Developmental Biology - Plant (2020)es_AR
dc.subjectMinthostachyseng
dc.subjectSomatic Embryogenesiseng
dc.subjectEmbriogénesis Somáticaes_AR
dc.subjectIn Vitro Cultureeng
dc.subjectCultivo in Vitroes_AR
dc.subject.otherGrisebes_AR
dc.subject.otherPeperinaes_AR
dc.titleHigh - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.filFil: Goytia Bertero, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas Químicas y Naturales. Cátedra de Ingeniería Genética; Argentinaes_AR
dc.description.filFil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentinaes_AR
dc.description.filFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentinaes_AR
dc.description.filFil: Arteaga, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentinaes_AR
dc.description.filFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentinaes_AR
dc.description.filFil: Auteri, Micol Tais. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina
dc.subtypecientifico


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