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In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, [ver mas...]
dc.contributor.authorRapoport, Daria
dc.contributor.authorPatrmanova, T.
dc.contributor.authorKopecky, J.
dc.contributor.authorMareckova, M.
dc.contributor.authorClemente, Gladys
dc.contributor.authorSalvalaggio, Andrea Eugenia
dc.date.accessioned2020-10-13T17:53:55Z
dc.date.available2020-10-13T17:53:55Z
dc.date.issued2020-01
dc.identifier.issn1406-894X
dc.identifier.otherhttps://doi.org/10.15159/ar.20.157
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8039
dc.identifier.urihttps://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf
dc.description.abstractIn this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherEstonian Agricultural Universityes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceAgronomy Research 18 (S2) : 1433–1443 (2020)es_AR
dc.subjectPapaes_AR
dc.subjectPotatoeseng
dc.subjectStreptomyces Scabieses_AR
dc.subjectEnfermedades Bacterianases_AR
dc.subjectBacterial Diseaseseng
dc.subjectAmérica del Sures_AR
dc.subjectSouth Americaeng
dc.subject.otherSarna Comúnes_AR
dc.titleIsolation of streptomycetes causing common scab from 3-years old potato samples from South Americaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Rapoport, Daria. Charles University in Prague. Faculty opf Science, República Checa. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.es_AR
dc.description.filFil: Patrmanova, T. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.es_AR
dc.description.filFil: Kopecky, J. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa.es_AR
dc.description.filFil: Mareckova, M. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Czech University of Life Sciences, Faculty of Agrobiology, Food, and Natural Resources, Department of Microbiology, República Checa.es_AR
dc.description.filFil: Clemente, Gladys. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.subtypecientifico


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