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Resumen
Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined [ver mas...]
dc.contributor.authorde Felice, Lorena Alejandra
dc.contributor.authorMoré, Gastón
dc.contributor.authorCappuccio, Javier Alejandro
dc.contributor.authorVenturini, María Cecilia
dc.contributor.authorUnzaga, Juan Manuel
dc.date.accessioned2020-09-30T13:13:54Z
dc.date.available2020-09-30T13:13:54Z
dc.date.issued2020-09
dc.identifier.issn2405-9390
dc.identifier.otherhttps://doi.org/10.1016/j.vprsr.2020.100473
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7991
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S2405939020302549
dc.description.abstractCryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Parasitology: Regional Studies and Reports 22 : 100473 (December 2020)es_AR
dc.subjectCerdoes_AR
dc.subjectSwineeng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectCryptosporidiumes_AR
dc.subjectOocistoses_AR
dc.subjectOocystseng
dc.subjectLechónes_AR
dc.subjectPigletseng
dc.subjectArgentina
dc.subject.otherCryptosporidium suises_AR
dc.subject.otherCryptosporidium scrofarumes_AR
dc.titleMolecular characterization of Cryptosporidium spp. from domestic pigs in Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Marcos Juárezes_AR
dc.description.filFil: de Felice, Lorena A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentinaes_AR
dc.description.filFil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentinaes_AR
dc.description.filFil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentinaes_AR
dc.subtypecientifico


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