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Abstract
Entomopathogens help regulate natural insect populations, and their use in pest management is the subject of active research. Classical and molecular techniques are currently available to elucidate disease etiology and pathogenesis. Here, we performed an Azan trichrome stain for light microscopic visualization of pathogens in the larval tissues of two Neotropical lepidopterans. Epinotia aporema larvae exhibiting symptoms of microsporidiosis were [ver mas...]
dc.contributor.authorArneodo Larochette, Joel Demian
dc.contributor.authorTaibo, Catalina Beatriz
dc.contributor.authorSciocco, Alicia Ines
dc.date.accessioned2017-07-26T15:31:20Z
dc.date.available2017-07-26T15:31:20Z
dc.date.issued2016
dc.identifier.issn1851-7617
dc.identifier.otherhttp://dx.doi.org/10.1016/j.ram.2016.04.008
dc.identifier.urihttp://hdl.handle.net/20.500.12123/785
dc.identifier.urihttp://www.scielo.org.ar/pdf/ram/v48n4/v48n4a17.pdf
dc.identifier.urihttp://ac.els-cdn.com/S0325754116300505/1-s2.0-S0325754116300505-main.pdf?_tid=23199db6-720f-11e7-8d75-00000aab0f27&acdnat=1501079679_d9fb0fa263a66265937d595ac24e3e5a
dc.description.abstractEntomopathogens help regulate natural insect populations, and their use in pest management is the subject of active research. Classical and molecular techniques are currently available to elucidate disease etiology and pathogenesis. Here, we performed an Azan trichrome stain for light microscopic visualization of pathogens in the larval tissues of two Neotropical lepidopterans. Epinotia aporema larvae exhibiting symptoms of microsporidiosis were fortuitously detected in a rearing facility (IMyZA-INTA, Argentina), while Helicoverpa gelotopoeon specimens were experimentally infected with an Argentinean isolate of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Caterpillars were fixed in Duboscq-Brasil and paraffin embedded. Microtome sections (5 μm-thick) were deparaffinized and stained2 for observation under a Nikon Eclipse 80i microscope equipped with a Nikon DS-U3 digital camera.
dc.formatapplication/pdf
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista Argentina de Microbiología 48 (4) : 358-359
dc.subjectPlagas de plantas
dc.subjectPest of Plantseng
dc.subjectMicroscopia
dc.subjectMicroscopyeng
dc.subjectEpinotia aporema
dc.subjectHelicoverpa
dc.subject.otherEntomopatógenos
dc.titleLight microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.description.origenInst. de Microbiología y Zoología Agrícola IMyZA
dc.gic152531
dc.description.filFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Servicio de Microscopía; Argentina
dc.description.filFil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
dc.subtypecientifico


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